Cell No. : Cell Name
AES0121 : Nanog^(βgeo/+)ES
update : 2022/12/21
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| Comment | A subline of mouse embryonic stem cell (RF8 derived from 129 SV Jae strain mouse). Nanog gene was replaced with βgeo gene in one allele. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
https://www.cira.kyoto-u.ac.jp/
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| Originator |
Yamanaka, Shinya
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| Year of deposit |
2007
|
| Another name |
RCB2662
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| Animal |
_mouse
< Mammals
|
| Strain name |
129 SV Jae
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| Gender |
Male
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells
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| Classification |
ES
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| Recombinant |
recombinant
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| Exogene |
βgeo gene
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_T795
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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2 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1-2 x 10 6 cells/100 mm dish
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
0
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