Cell No. : Cell Name AES0119 : EBRTcH3(serum)  update : 2023/07/19
Comment			Mouse embryonic stem (ES) cell line derived from 129/Ola mouse. Feeder-free cell line
Comment from the depositor
Terms and conditions			In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nucleic Acids Res 2005;33:e43) designated by the DEPOSITOR is required.
Remarks
Order Form			Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Masui, Shinji
		Originator	Masui, Shinji
		Year of deposit	2007
		Another name	RCB2659
		Animal	_mouse < Mammals
		Strain name	129/Ola
		Gender	Male
		Age at sampling	embryo
		Tissue	embryonic stem cells
		Classification	ES
		Recombinant	recombinant
		Exogene	pMW ROSATcH, IRES-BSDpA
		Lifespan	infinite
		Morphology	ES-like
		Cellosaurus(Expasy)	CVCL_U953
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		GMEM + 10% FBS (Stem Cell Science KK, Tetracycline Screened FBS) + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
		Antibiotics		Free
		Passage method		0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
		Coating dish		0.1% gelatin coated dish
	Culture information	Passage cell No		3-5 x 10 5 cells/60 mm dish
		SC frequency		Subculture : once/2-3 days
		Temperature		37 ℃
		CO2 concentration		5 %
		Feeder cells		free
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		Others		Selection medium : Medium+10μg/ml Blasticidin+100μg/ml Hygromycin
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	0

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Reference

4585  Matoba R, Niwa H, Masui S, Ohtsuka S, Carter MG, Sharov AA, Ko MS.  Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling.  PLoS One  2006  1:e26.  PubMed ID: 17183653   DOI: 10.1371/journal.pone.0000026

4583  Masui S, Shimosato D, Toyooka Y, Yagi R, Takahashi K, Niwa H.  An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit.  Nucleic Acids Res  2005  33(4):e43  PubMed ID: 15741176   DOI: 10.1093/nar/gni043

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