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Cell No. : Cell Name
AES0119 : EBRTcH3(serum)  update : 2018/07/06
CommentMouse embryonic stem (ES) cell line derived from 129/Ola mouse. Feeder-free cell line
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nucleic Acids Res 2005;33:e43) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Masui, Shinji
Originator Masui, Shinji
Year of deposit 2007
Another name RCB2659
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene pMW ROSATcH, IRES-BSDpA
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 10% FBS (Stem Cell Science KK, Tetracycline Screened FBS) + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Others Selection medium : Medium+10μg/ml Blasticidin+100μg/ml Hygromycin
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
4585  Matoba R, Niwa H, Masui S, Ohtsuka S, Carter MG, Sharov AA, Ko MS.  Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling.  PLoS One  2006  1:e26.  PubMed ID: 17183653
4583  Masui S, Shimosato D, Toyooka Y, Yagi R, Takahashi K, Niwa H.  An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit.  Nucleic Acids Res  2005  33(4):e43  PubMed ID: 15741176

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