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Cell No. : Cell Name
AES0118 : NOD・ESA  update : 2023/04/11
CommentMouse embryonic stem (ES) cell line derived from NOD mouse.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Kyusyu University

3-1-1 Maidashi, Higashi-ku, Fukuoka Japan
Dr.NAGAFUCHI Seiho
Fax. +81-92-642-6731
Order Form Order Form(C-0032.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nagafuchi, Seiho
Originator Nagafuchi, Seiho
Year of deposit 2007
Another name RCB2608
Animal _mouse < Mammals
Strain name NOD
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_U868
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose, 1mM Sodium Pyruvate) + 20% FBS + 0.05mM NEAA + Nucleosides (ES Cell Qualified) + 0.055mM 2-Mercaptoethanol + 10000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10 5 cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF (BALB/c)
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10 5 cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 1


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Reference
4590  Arai S, Minjares C, Nagafuchi S, Miyazaki T.  Improved experimental procedures for achieving efficient germ line transmission of nonobese diabetic (NOD)-derived embryonic stem cells.  Exp Diabesity Res  2004  5(3):219-26  PubMed ID: 15512790   DOI: 10.1080/15438600490486877
4589  Nagafuchi S, Katsuta H, Kogawa K, Akashi T, Kondo S, Sakai Y, Tsukiyama T, Kitamura D, Niho Y, Watanabe T.  Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmission.  FEBS Lett  1999  455(1-2):101-4.  PubMed ID: 10428481   DOI: 10.1016/s0014-5793(99)00801-7

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User's Publication
4591  Ikeda T, Hirata S, Takamatsu K, Haruta M, Tsukamoto H, Ito T, Uchino M, Ando Y, Nagafuchi S, Nishimura Y, Senju S.  Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells.  PLoS One  2014  9(12):e115198  PubMed ID: 25522369   DOI: 10.1371/journal.pone.0115198


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