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Cell No. : Cell Name
AES0054 : EBRTcH3  update : 2023/07/19
CommentMouse embryonic stem cell line. Convenient to establish the line carrying an inducible expression unit (see reference). Feeder-free cell line.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nucleic Acids Res 2005;33:e43) designated by the DEPOSITOR is required.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Masui, Shinji
Originator Masui, Shinji
Year of deposit 2006
Another name RCB2325
Animal _mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_J649
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium CultiCell + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 5-10 x 10 5 cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Others Selection medium : Medium+10μg/ml Blasticidin+100μg/ml Hygromycin
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lot info
Reference information Reference 2
User's Publication 2

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4585  Matoba R, Niwa H, Masui S, Ohtsuka S, Carter MG, Sharov AA, Ko MS.  Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling.  PLoS One  2006  1:e26.  PubMed ID: 17183653   DOI: 10.1371/journal.pone.0000026
4583  Masui S, Shimosato D, Toyooka Y, Yagi R, Takahashi K, Niwa H.  An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit.  Nucleic Acids Res  2005  33(4):e43  PubMed ID: 15741176   DOI: 10.1093/nar/gni043

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User's Publication
11289  Hirota A, Nakajima-Koyama M, Ashida Y, Nishida E.  The nucleosome remodeling and deacetylase complex protein CHD4 regulates neural differentiation of mouse embryonic stem cells by down-regulating p53.  J. Biol. Chem.  2019    PubMed ID: 30409903   DOI: 10.1074/jbc.RA118.004086
14134  Yano Y, Iimura N, Kojima N, Uchiyama H.  Non-neural and cardiac differentiating properties of Tbx6-expressing mouse embryonic stem cells  Regen Ther   2016  3:1-6  PubMed ID: 31245465   DOI: 10.1016/j.reth.2016.02.001

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