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Cell No. : Cell Name
AES0033 : B6mt-1  update : 2023/06/30
CommentMouse embryonic stem (ES) cell line established by nuclear transfer technique. Nucleus was derived from C57BL/6 male mouse. Feeder-free cell line.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Science 2001;292:740-743) designated by the DEPOSITOR is required.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Wakayama, Teruhiko
Originator Wakayama, Teruhiko
Year of deposit 2005
Another name RCB2182
Animal _mouse < Mammals
Strain name C57BL/6: nucleus
Gender Male
Age at sampling 2-3 months
Tissue tail (possibly fibroblast)
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_F034
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (knockout, 2mM L-Glutamine) + 20% FBS + Nucleosides (ES Cell Qualified) + 0.1mM NEAA (ES Cell Qualified) + 0.05mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 5-7 x 10 5 cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 1

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3499  Wakayama S, Jakt ML, Suzuki M, Araki R, Hikichi T, Kishigami S, Ohta H, Van Thuan N, Mizutani E, Sakaide Y, Senda S, Tanaka S, Okada M, Miyake M, Abe M, Nishikawa S, Shiota K, Wakayama T.  Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts.  Stem Cells  2006  24(9):2023-33  PubMed ID: 16690779   DOI: 10.1634/stemcells.2005-0537
3497  Wakayama S, Ohta H, Kishigami S, Thuan NV, Hikichi T, Mizutani E, Miyake M, Wakayama T.  Establishment of male and female nuclear transfer embryonic stem cell lines from different mouse strains and tissues.  Biol Reprod  2005  72(4):932-6  PubMed ID: 15601921   DOI: 10.1095/biolreprod.104.035105
3492  Wakayama T, Tabar V, Rodriguez I, Perry AC, Studer L, Mombaerts P.  Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer.  Science  2001  92(5517):740-3  PubMed ID: 11326103   DOI: 10.1126/science.1059399

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User's Publication
7072  Kunitomi A, Yuasa S, Sugiyama F, Saito Y, Seki T, Kusumoto D, Kashimura S, Takei M, Tohyama S, Hashimoto H, Egashira T, Tanimoto Y, Mizuno S, Tanaka S, Okuno H, Yamazawa K, Watanabe H, Oda M, Kaneda R, Matsuzaki Y, Nagai T, Okano H, Yagami K, Tanaka M, Fukuda K.  H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells.  Stem Cell Reports  2016  6:825-33  PubMed ID: 27237376   DOI: 10.1016/j.stemcr.2016.04.015

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