Cell No. : Cell Name
AES0033 : B6mt-1 update : 2023/06/30 |
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Comment | Mouse embryonic stem (ES) cell line established by nuclear transfer technique. Nucleus was derived from C57BL/6 male mouse. Feeder-free cell line. | |||
Comment from the depositor | ||||
Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Science 2001;292:740-743) designated by the DEPOSITOR is required. | |||
Remarks | ||||
Order Form | Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | Wakayama, Teruhiko | ||
Originator | Wakayama, Teruhiko | |||
Year of deposit | 2005 | |||
Another name | RCB2182 | |||
Animal | _mouse < Mammals | |||
Strain name | C57BL/6: nucleus | |||
Gender | Male | |||
Age at sampling | 2-3 months | |||
Tissue | tail (possibly fibroblast) | |||
Classification | ES | Lifespan | infinite | |
Morphology | ES-like | |||
Cellosaurus(Expasy) | CVCL_F034 | |||
Medium | Medium List | Culture type | Adherent cells | |
Culture medium | DMEM (knockout, 2mM L-Glutamine) + 20% FBS + Nucleosides (ES Cell Qualified) + 0.1mM NEAA (ES Cell Qualified) + 0.05mM 2-Mercaptoethanol + 1000U/ml mouse LIF | |||
Antibiotics | Free | Passage method | 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA) | |
Coating dish | 0.1% gelatin coated dish | Culture information | Passage cell No | 5-7 x 10 5 cells/60 mm dish |
SC frequency | Subculture : once/2-3 days | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Feeder cells | free | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Isozyme | LD, NP | |||
Images | ||||
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Reference information | Reference | 3 | ||
User's Publication | 1 | |||