Cell No. : Cell Name
AES0026 : BD129mt-2
update : 2023/06/30
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| Comment | Mouse embryonic stem (ES) cell line established by nuclear transfer technique. Nucleus was derived from F1 male mouse ((C57BL/6 x DBA2) x 129/Sv). Feeder-free cell line. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Science 2001;292:740-743) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Wakayama, Teruhiko
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| Originator |
Wakayama, Teruhiko
|
| Year of deposit |
2005
|
| Another name |
RCB2175
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| Animal |
_mouse
< Mammals
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| Strain name |
F1(C57BL/6xDBA2)x129/Sv): nucleus
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| Gender |
Male
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| Age at sampling |
2-3 months
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| Tissue |
tail (possibly fibroblast)
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| Classification |
ES
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_C329
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (knockout, 2mM L-Glutamine) + 20% FBS + Nucleosides (ES Cell Qualified) + 0.1mM NEAA (ES Cell Qualified) + 0.05mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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5-7 x 10 5 cells/60 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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free
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
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| User's Publication |
0
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| Reference |
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3499
Wakayama S, Jakt ML, Suzuki M, Araki R, Hikichi T, Kishigami S, Ohta H, Van Thuan N, Mizutani E, Sakaide Y, Senda S, Tanaka S, Okada M, Miyake M, Abe M, Nishikawa S, Shiota K, Wakayama T.
Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts.
Stem Cells
2006
24(9):2023-33
PubMed ID: 16690779
DOI: 10.1634/stemcells.2005-0537
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3497
Wakayama S, Ohta H, Kishigami S, Thuan NV, Hikichi T, Mizutani E, Miyake M, Wakayama T.
Establishment of male and female nuclear transfer embryonic stem cell lines from different mouse strains and tissues.
Biol Reprod
2005
72(4):932-6
PubMed ID: 15601921
DOI: 10.1095/biolreprod.104.035105
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3498
Wakayama S, Mizutani E, Kishigami S, Thuan NV, Ohta H, Hikichi T, Bui HT, Miyake M, Wakayama T.
Mice cloned by nuclear transfer from somatic and ntES cells derived from the same individuals.
J Reprod Dev.
2005
51(6):765-72
PubMed ID: 16227673
DOI: 10.1262/jrd.17061
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3492
Wakayama T, Tabar V, Rodriguez I, Perry AC, Studer L, Mombaerts P.
Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer.
Science
2001
92(5517):740-3
PubMed ID: 11326103
DOI: 10.1126/science.1059399
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