Cell No. : Cell Name AES0020 : BCmt-1  update : 2023/06/30
Comment			Mouse embryonic stem (ES) cell line established by nuclear transfer technique. Nucleus was derived from F1 male mouse (C57BL/6 x C3H/He). Feeder-free cell line
Comment from the depositor
Terms and conditions			In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Science 2001;292:740-743) designated by the DEPOSITOR is required.
Remarks
Order Form			Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Wakayama, Teruhiko
		Originator	Wakayama, Teruhiko
		Year of deposit	2005
		Another name	RCB2169
		Animal	_mouse < Mammals
		Strain name	F1(C57BL/6xC3H/He): nucleus
		Gender	Male
		Age at sampling	2-3 months
		Tissue	tail (possibly fibroblast)
		Classification	ES
		Lifespan	infinite
		Morphology	ES-like
		Cellosaurus(Expasy)	CVCL_C324
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (knockout, 2mM L-Glutamine) + 20% FBS + Nucleosides (ES Cell Qualified) + 0.1mM NEAA (ES Cell Qualified) + 0.05mM 2-Mercaptoethanol + 1000U/ml mouse LIF
		Antibiotics		Free
		Passage method		0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
		Coating dish		0.1% gelatin coated dish
	Culture information	Passage cell No		5-7 x 10 5 cells/60 mm dish
		SC frequency		Subculture : once/2-3 days
		Temperature		37 ℃
		CO2 concentration		5 %
		Feeder cells		free
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	0

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Reference

3499  Wakayama S, Jakt ML, Suzuki M, Araki R, Hikichi T, Kishigami S, Ohta H, Van Thuan N, Mizutani E, Sakaide Y, Senda S, Tanaka S, Okada M, Miyake M, Abe M, Nishikawa S, Shiota K, Wakayama T.  Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts.  Stem Cells  2006  24(9):2023-33  PubMed ID: 16690779   DOI: 10.1634/stemcells.2005-0537

3497  Wakayama S, Ohta H, Kishigami S, Thuan NV, Hikichi T, Mizutani E, Miyake M, Wakayama T.  Establishment of male and female nuclear transfer embryonic stem cell lines from different mouse strains and tissues.  Biol Reprod  2005  72(4):932-6  PubMed ID: 15601921   DOI: 10.1095/biolreprod.104.035105

3492  Wakayama T, Tabar V, Rodriguez I, Perry AC, Studer L, Mombaerts P.  Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer.  Science  2001  92(5517):740-3  PubMed ID: 11326103   DOI: 10.1126/science.1059399

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