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Cell No. : Cell Name
AES0016 : BDmt-2  update : 2023/06/30
CommentMouse embryonic stem (ES) cell line established by nuclear transfer technique. Nucleus was derived from F1 male mouse (C57BL/6 x DBA2). Feeder-free cell line.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Science 2001;292:740-743) designated by the DEPOSITOR is required.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
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Basic information Depositor Wakayama, Teruhiko
Originator Wakayama, Teruhiko
Year of deposit 2005
Another name RCB2165
Animal _mouse < Mammals
Strain name F1(C57BL/6xDBA2): nucleus
Gender Male
Age at sampling 2-3 months
Tissue tail (possibly fibroblast)
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_C333
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (knockout, 2mM L-Glutamine) + 20% FBS + Nucleosides (ES Cell Qualified) + 0.1mM NEAA (ES Cell Qualified) + 0.05mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 5-7 x 10 5 cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
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lot info
Reference information Reference 3
User's Publication 0

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3499  Wakayama S, Jakt ML, Suzuki M, Araki R, Hikichi T, Kishigami S, Ohta H, Van Thuan N, Mizutani E, Sakaide Y, Senda S, Tanaka S, Okada M, Miyake M, Abe M, Nishikawa S, Shiota K, Wakayama T.  Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts.  Stem Cells  2006  24(9):2023-33  PubMed ID: 16690779   DOI: 10.1634/stemcells.2005-0537
3497  Wakayama S, Ohta H, Kishigami S, Thuan NV, Hikichi T, Mizutani E, Miyake M, Wakayama T.  Establishment of male and female nuclear transfer embryonic stem cell lines from different mouse strains and tissues.  Biol Reprod  2005  72(4):932-6  PubMed ID: 15601921   DOI: 10.1095/biolreprod.104.035105
3492  Wakayama T, Tabar V, Rodriguez I, Perry AC, Studer L, Mombaerts P.  Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer.  Science  2001  92(5517):740-3  PubMed ID: 11326103   DOI: 10.1126/science.1059399

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