Cell No. : Cell Name
AES0014 : TT2
update : 2023/04/11
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| Comment | Mouse ES cell line (B6 X CBA). This cell line can be used only for in vitro culture experiment. |
| Comment from the depositor | |
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Anal Biochem 1993;214:70-76). 2) Remarks:Germline-differentiating potency is not guaranteed. The TT2 cells with high germline-differentiating potency will be distributed by Laboratory for Animal Resources and Genetic Engineering (LARGE:mutant@cdb.riken.jp), RIKEN Center for Biosystems Dynamics Research. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Aizawa, Shinichi
|
| Originator |
Tokunaga, T. & Aizawa, S.
|
| Year of deposit |
2005
|
| Another name |
RCB2077
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57BL/6 x CBA
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Classification |
ES
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_C318
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deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
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Adherent cells
|
| Culture medium |
|
DMEM (high glucose, 1mM Sodium Pyruvate) + 20% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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3-5 x 10 5 cells/60 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
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MEF
|
| Feeder treatment |
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X-rays 5000R (or MMC)
|
| Feeder seed cells |
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2.5-5.0 x 10 5 cells/60 mm dish
|
| Freeze medium |
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Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
6
|
| Reference |
|
3268
Yagi T, Tokunaga T, Furuta Y, Nada S, Yoshida M, Tsukada T, Saga Y, Takeda N, Ikawa Y, Aizawa S
A novel ES cell line, TT2, with high germline-differentiating potency.
Anal Biochem
1993
214:70-6
PubMed ID: 8250257
DOI: 10.1006/abio.1993.1458
|
|
17891
Saga Y, Yagi T, Ikawa Y, Sakakura T, Aizawa S.
Mice develop normally without tenascin
Genes Dev
1992
6(10):1821-31
PubMed ID: 1383086
DOI: 10.1101/gad.6.10.1821
|
| User's Publication |
|
21640
Lin YC, Wuputra K, Kato K, Ku CC, Saito S, Noguchi M, Nakamura Y, Hsiao M, Lin CS, Wu DC, Kawaguchi A, Yu HS, Yokoyama KK.
Di-n-butyl phthalate promotes the neural differentiation of mouse embryonic stem cells through neurogenic differentiation 1.
Environ Pollut
2024
123722
PubMed ID: 38460589
DOI: 10.1016/j.envpol.2024.123722
|
|
21223
Li J, Bai Y, Liu Y, Song Z, Yang Y, Zhao Y.
Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems.
Cell Rep
2023
42(6):112566
PubMed ID: 37235474
DOI: 10.1016/j.celrep.2023.112566
|
|
11168
Tsunoda T, Doi K, Ishikura S, Luo H, Nishi K, Matsuzaki H, Koyanagi M, Tanaka Y, Okamura T, Shirasawa S.
Zfat expression in ZsGreen reporter gene knock-in mice: Implications for a novel function of Zfat in definitive erythropoiesis.
Int. J. Mol. Med.
2018
42(5):2595-2603
PubMed ID: 30106088
DOI: 10.3892/ijmm.2018.3806
|
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10256
Yang Y, Liu B, Xu J, Wang J, Wu J, Shi C, Xu Y, Dong J, Wang C, Lai W, Zhu J, Xiong L, Zhu D, Li X, Yang W, Yamauchi T, Sugawara A, Li Z, Sun F, Li X, Li C, He A, Du Y, Wang T, Zhao C, Li H, Chi X, Zhang H, Liu Y, Li C, Duo S, Yin M, Shen H, Belmonte JCI, Deng H.
Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency.
Cell
2017
169:243-257.e25
PubMed ID: 28388409
DOI: 10.1016/j.cell.2017.02.005
|
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9579
Kanegi R, Hatoya S, Tsujimoto Y, Takenaka S, Nishimura T, Wijewardana V, Sugiura K, Takahashi M, Kawate N, Tamada H, Inaba T.
Production of feline leukemia inhibitory factor with biological activity in Escherichia coli.
Theriogenology
2016
86:604-11
PubMed ID: 27020881
DOI: 10.1016/j.theriogenology.2016.02.013
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5313
Ishisaka A, Mukai R, Terao J, Shibata N, Kawai Y.
Specific localization of quercetin-3-O-glucuronide in human brain.
Arch Biochem Biophys
2014
557:11-7
PubMed ID: 24893148
DOI: 10.1016/j.abb.2014.05.025
|
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