Cell No. : Cell Name AES0014 : TT2  update : 2023/01/05
Comment			Mouse ES cell line (B6 X CBA). This cell line can be used only for in vitro culture experiment.
Comment from the depositor
Terms and conditions			1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Anal Biochem 1993;214:70-76). 2) Remarks:Germline-differentiating potency is not guaranteed. The TT2 cells with high germline-differentiating potency will be distributed by Laboratory for Animal Resources and Genetic Engineering (LARGE:mutant@cdb.riken.jp), RIKEN Center for Biosystems Dynamics Research.
Remarks
Order Form			Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Aizawa, Shinichi
		Originator	Tokunaga, T. & Aizawa, S.
		Year of deposit	2005
		Another name	RCB2077
		Animal	mouse < Mammals
		Strain name	C57BL/6 x CBA
		Gender	Male
		Age at sampling	embryo
		Tissue	embryonic stem cells
		Classification	ES
		Lifespan	infinite
		Morphology	ES-like
		Cellosaurus(Expasy)	CVCL_C318
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Medium and additives		DMEM (high glucose, 1mM Sodium Pyruvate) + 20% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
		Antibiotics		Free
		Passage method		0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
		Coating dish		0.1% gelatin coated dish
	Culture information	Passage cell No		3-5 x 10^(5) cells/60 mm dish
		SC frequency		Subculture : once/2-3 days
		Temperature		37 ℃
		CO2 concentration		5 %
		Feeder cells		MEF
		Feeder treatment		X-rays 5000R (or MMC)
		Feeder seed cells		2.5-5.0 x 10^(5) cells/60 mm dish
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	4

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Reference

3268  Yagi T, Tokunaga T, Furuta Y, Nada S, Yoshida M, Tsukada T, Saga Y, Takeda N, Ikawa Y, Aizawa S  A novel ES cell line, TT2, with high germline-differentiating potency.  Anal Biochem  1993  214:70-6  PubMed ID: 8250257   DOI: 10.1006/abio.1993.1458

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User's Publication

11168  Tsunoda T, Doi K, Ishikura S, Luo H, Nishi K, Matsuzaki H, Koyanagi M, Tanaka Y, Okamura T, Shirasawa S.  Zfat expression in ZsGreen reporter gene knock-in mice: Implications for a novel function of Zfat in definitive erythropoiesis.  Int. J. Mol. Med.  2018  42(5):2595-2603  PubMed ID: 30106088   DOI: 10.3892/ijmm.2018.3806

10256  Yang Y, Liu B, Xu J, Wang J, Wu J, Shi C, Xu Y, Dong J, Wang C, Lai W, Zhu J, Xiong L, Zhu D, Li X, Yang W, Yamauchi T, Sugawara A, Li Z, Sun F, Li X, Li C, He A, Du Y, Wang T, Zhao C, Li H, Chi X, Zhang H, Liu Y, Li C, Duo S, Yin M, Shen H, Belmonte JCI, Deng H.  Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency.  Cell  2017  169:243-257.e25  PubMed ID: 28388409   DOI: 10.1016/j.cell.2017.02.005

9579  Kanegi R, Hatoya S, Tsujimoto Y, Takenaka S, Nishimura T, Wijewardana V, Sugiura K, Takahashi M, Kawate N, Tamada H, Inaba T.  Production of feline leukemia inhibitory factor with biological activity in Escherichia coli.  Theriogenology  2016  86:604-11  PubMed ID: 27020881   DOI: 10.1016/j.theriogenology.2016.02.013

5313  Ishisaka A, Mukai R, Terao J, Shibata N, Kawai Y.  Specific localization of quercetin-3-O-glucuronide in human brain.  Arch Biochem Biophys  2014  557:11-7  PubMed ID: 24893148   DOI: 10.1016/j.abb.2014.05.025