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Cell No. : Cell Name
AES0014 : TT2  update : 2023/04/11
CommentMouse ES cell line (B6 X CBA). This cell line can be used only for in vitro culture experiment.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Anal Biochem 1993;214:70-76). 2) Remarks:Germline-differentiating potency is not guaranteed. The TT2 cells with high germline-differentiating potency will be distributed by Laboratory for Animal Resources and Genetic Engineering (, RIKEN Center for Biosystems Dynamics Research.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Aizawa, Shinichi
Originator Tokunaga, T. & Aizawa, S.
Year of deposit 2005
Another name RCB2077
Animal _mouse < Mammals
Strain name C57BL/6 x CBA
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_C318
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose, 1mM Sodium Pyruvate) + 20% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10 5 cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10 5 cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 6

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3268  Yagi T, Tokunaga T, Furuta Y, Nada S, Yoshida M, Tsukada T, Saga Y, Takeda N, Ikawa Y, Aizawa S  A novel ES cell line, TT2, with high germline-differentiating potency.  Anal Biochem  1993  214:70-6  PubMed ID: 8250257   DOI: 10.1006/abio.1993.1458
17891  Saga Y, Yagi T, Ikawa Y, Sakakura T, Aizawa S.  Mice develop normally without tenascin  Genes Dev  1992  6(10):1821-31  PubMed ID: 1383086   DOI: 10.1101/gad.6.10.1821

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User's Publication
21640  Lin YC, Wuputra K, Kato K, Ku CC, Saito S, Noguchi M, Nakamura Y, Hsiao M, Lin CS, Wu DC, Kawaguchi A, Yu HS, Yokoyama KK.  Di-n-butyl phthalate promotes the neural differentiation of mouse embryonic stem cells through neurogenic differentiation 1.  Environ Pollut  2024  123722  PubMed ID: 38460589   DOI: 10.1016/j.envpol.2024.123722
21223  Li J, Bai Y, Liu Y, Song Z, Yang Y, Zhao Y.  Transcriptome-based chemical screens identify CDK8 as a common barrier in multiple cell reprogramming systems.  Cell Rep  2023  42(6):112566  PubMed ID: 37235474   DOI: 10.1016/j.celrep.2023.112566
11168  Tsunoda T, Doi K, Ishikura S, Luo H, Nishi K, Matsuzaki H, Koyanagi M, Tanaka Y, Okamura T, Shirasawa S.  Zfat expression in ZsGreen reporter gene knock-in mice: Implications for a novel function of Zfat in definitive erythropoiesis.  Int. J. Mol. Med.  2018  42(5):2595-2603  PubMed ID: 30106088   DOI: 10.3892/ijmm.2018.3806
10256  Yang Y, Liu B, Xu J, Wang J, Wu J, Shi C, Xu Y, Dong J, Wang C, Lai W, Zhu J, Xiong L, Zhu D, Li X, Yang W, Yamauchi T, Sugawara A, Li Z, Sun F, Li X, Li C, He A, Du Y, Wang T, Zhao C, Li H, Chi X, Zhang H, Liu Y, Li C, Duo S, Yin M, Shen H, Belmonte JCI, Deng H.  Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency.  Cell  2017  169:243-257.e25  PubMed ID: 28388409   DOI: 10.1016/j.cell.2017.02.005
9579  Kanegi R, Hatoya S, Tsujimoto Y, Takenaka S, Nishimura T, Wijewardana V, Sugiura K, Takahashi M, Kawate N, Tamada H, Inaba T.  Production of feline leukemia inhibitory factor with biological activity in Escherichia coli.  Theriogenology  2016  86:604-11  PubMed ID: 27020881   DOI: 10.1016/j.theriogenology.2016.02.013
5313  Ishisaka A, Mukai R, Terao J, Shibata N, Kawai Y.  Specific localization of quercetin-3-O-glucuronide in human brain.  Arch Biochem Biophys  2014  557:11-7  PubMed ID: 24893148   DOI: 10.1016/

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