Cell No. : Cell Name
update : 2023/04/11
Comment Nuclear transferred mouse embryonic stem cells. Nucleus was derived from (B6xDBA2) mouse.
Comment from the depositor
Terms and conditions A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
RIKEN, BioResource Research Center
Order Form
Order Form(C-0032.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see ). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp ).
Basic information
Depositor
Ogura, Atsuo
Originator
Hirose, Michiko
Year of deposit
2005
Another name
RCB2012
Animal
_mouse
< Mammals
Strain name
B6D2F1xB6D2F1
Gender
Female
Age at sampling
embryo
Tissue
embryonic stem cells
Classification
ES
Lifespan
infinite
Morphology
ES-like
Cellosaurus(Expasy)
CVCL_B986
deposit info lot info
Medium
Medium List
Culture type
Adherent cells
Culture medium
DMEM (high glucose, 1mM Sodium Pyruvate) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics
Free
Passage method
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish
0.1% gelatin coated dish
Culture information
Passage cell No
3-5 x 10 5 cells/60 mm dish
SC frequency
Subculture : once/2-3 days
Temperature
37
℃
CO2 concentration
5
%
Feeder cells
MEF
Feeder treatment
X-rays 5000R (or MMC)
Feeder seed cells
2.5-5.0 x 10 5 cells/60 mm dish
Freeze medium
Medium : 2 x FM1 = 1 : 1
Freezing method
Slow freezing
Mycoplasma/Acholeplasma
(-)
Isozyme
LD, NP
Reference information
Reference
0
User's Publication
0
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