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Cell No. : Cell Name
AES0010 : BRC6  update : 2023/01/04
CommentMouse embryonic stem cells derived from C57BL/6 embryo (female) .
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
RIKEN, BioResource Research Center

3-1-1 Koyadai,Tsukuba-shi, Ibaraki 305-0074 Japan
Dr.OGURA Atsuo
Fax. +81-29-836-9172
Order Form Order Form(C-0032.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ogura, Atsuo
Originator Hirose, Michiko
Year of deposit 2005
Another name RCB2009
Animal _mouse < Mammals
Strain name C57BL/6xC57BL/6
Gender Female
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_B983
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose, 1mM Sodium Pyruvate) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10 5 cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10 5 cells/60 mm dish
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 0
User's Publication 7

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User's Publication
4166  Tran THY, Fukuda A, Aizawa S, Bui PL, Hayashi Y, Nishimura K, Hisatake K.  Live cell imaging of X chromosome reactivation during somatic cell reprogramming.  Biochem Biophys Rep  2018    PubMed ID: 30094351   DOI: 10.1016/j.bbrep.2018.07.007
4000  Okada A, Tashiro K, Yamaguchi T, Kawabata K.  Selective Differentiation into Hematopoietic and Cardiac Cells from Pluripotent Stem Cells Based on the Expression of Cell Surface Markers.  Methods Mol Biol  2016  1341:181-95  PubMed ID: 26138986   DOI: 10.1007/7651_2015_232
3948  Tashiro K, Hirata N, Okada A, Yamaguchi T, Takayama K, Mizuguchi H, Kawabata K.  Expression of coxsackievirus and adenovirus receptor separates hematopoietic and cardiac progenitor cells in fetal liver kinase 1-expressing mesoderm.  Stem Cells Transl Med  2015  4(5):424-36  PubMed ID: 25762001   DOI: 10.5966/sctm.2014-0173
14984  Himeno T, Kamiya H, Naruse K, Cheng Z, Ito S, Shibata T, Kondo M, Kato J, Okawa T, Fujiya A, Suzuki H, Kito T, Hamada Y, Oiso Y, Isobe K, Nakamura J.  Angioblast Derived from ES Cells Construct Blood Vessels and Ameliorate Diabetic Polyneuropathy in Mice  J Diabetes Res  2015  2015:257230  PubMed ID: 25977928   DOI: 10.1155/2015/257230
15230  Ito S, Hirabayashi K, Moriishi K, Matsui Y, Moriya K, Koike K, Matsuura Y, Shiota K, Yagi S.  Novel sex-dependent differentially methylated regions are demethylated in adult male mouse livers  Biochem Biophys Res Commun  2015  462(4):332-8  PubMed ID: 25960295   DOI: 10.1016/j.bbrc.2015.04.137
15804  Hacisuleyman E, Goff LA, Trapnell C, Williams A, Henao-Mejia J, Sun L, McClanahan P, Hendrickson DG, Sauvageau M, Kelley DR, Morse M, Engreitz J, Lander ES, Guttman M, Lodish HF, Flavell R, Raj A, Rinn JL.  Topological organization of multichromosomal regions by the long intergenic noncoding RNA Firre  Nat Struct Mol Biol   2014  21(2):198-206  PubMed ID: 24463464   DOI: 10.1038/nsmb.2764
6105  Tashiro K, Omori M, Kawabata K, Hirata N, Yamaguchi T, Sakurai F, Takaki S, Mizuguchi H.  Inhibition of Lnk in mouse induced pluripotent stem cells promotes hematopoietic cell generation.  Stem Cells Dev  2012  21(18):3381-90  PubMed ID: 22738147   DOI: 10.1089/scd.2012.0100

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