Cell No. : Cell Name
AES0009 : BRC5
update : 2026/05/14
|
| Comment | Mouse embryonic stem cells derived from C57BL/6 embryo. The karyotype is not normal. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Ogura, Atsuo
|
| Originator |
Hirose, Michiko
|
| Year of deposit |
2005
|
| Another name |
RCB2008
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57BL/6xC57BL/6
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Classification |
ES
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| SDS |
SDS_DMSO(Japanese) 
|
| Cellosaurus(Expasy) |
CVCL_B982
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (high glucose, 1mM Sodium Pyruvate) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
| Coating dish |
|
0.1% gelatin coated dish
|
Culture information
|
Passage cell No |
|
3-5 x 10 5 cells/60 mm dish
|
| SC frequency |
|
Subculture : once/2-3 days
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
MEF
|
| Feeder treatment |
|
X-rays 5000R (or MMC)
|
| Feeder seed cells |
|
2.5-5.0 x 10 5 cells/60 mm dish
|
| Freeze medium |
|
Medium : 2 x FM1 = 1 : 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
Back
Top
CELL BANK Website