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Cell No. : Cell Name
AES0004 : mGS-DBA1  update : 2022/02/21
CommentMultipotent germline stem (mGS) cell line derived from mouse testis.
Comment from the depositor
Terms and conditions1) A permission of the approver (Dr.Shinohara, Mito or Dr.Shinohara, Takashi) is required. 2) The users should sign an MTA with Kyoto University. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell 2004;119:1001-1012) designated by the DEPOSITOR is required. 4) The BIOLOGICAL RESOURCE should not be used for commercial purposes.
Remarks
approver's address
×
English
Address
Department of Molecular Genetics
Kyoto University Graduate School of Medicine
53 ShogoinKawaramachi, Sakyo-ku, Kyoto 606-8507, Japan
Dr.SHINOHARA Mito
Fax. +81-75-751-4169



English
Address
Department of Molecular Genetics
Kyoto University Graduate School of Medicine
53 ShogoinKawaramachi, Sakyo-ku, Kyoto 606-8507, JAPAN
Dr.SHINOHARA Takashi
Fax. +81-75-751-4169
Order Form Order Form(C-0032.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Shinohara, Mito
Originator Shinohara, Mito
Year of deposit 2005
Another name RCB2003
Animal mouse < Mammals
Strain name DBA/2
Gender Male
Age at sampling 1 day
Tissue testis
Classification ES
Year of origin 2004
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_1T53
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 15% FBS + 0.05mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 0.5-1.0 x 10^(6) cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
2838  Kanatsu-Shinohara, Mito, Inoue, Kimiko, Lee, Jiyoung, Yoshimoto, Momoko, Ogonuki, Narumi, Miki, Hiromi, Baba, Shiro, Kato, Takeo, Kazuki, Yasuhiro, Toyokuni, Shinya, Toyoshima, Megumi, Niwa, Ohtsura, Oshimura, Mitsuo, Heike, Toshio, Nakahata, Tatsutoshi, Ishino, Fumitoshi, Ogura, Atsuo, Shinohara, Takashi  Generation of pluripotent stem cells from neonatal mouse testis.  Cell  2004  119:1001-12  PubMed ID: 15620358   DOI: 10.1016/j.cell.2004.11.011

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User's Publication
10472  Umemura Y, Koike N, Ohashi M, Tsuchiya Y, Meng QJ, Minami Y, Hara M, Hisatomi M, Yagita K.  Involvement of posttranscriptional regulation of Clock in the emergence of circadian clock oscillation during mouse development.  Proc. Natl. Acad. Sci. U.S.A.  2017  114:E7479-E7488  PubMed ID: 28827343   DOI: 10.1073/pnas.1703170114



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