細胞番号 : 細胞名
AES0003 : B6G-2
update : 2024/10/10
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| 細胞特性(Comment:英) | Embryonic stem (ES) cell line derived from C57BL/6 mouse. Expressing GFP. |
| 細胞特性(日) | C57BL/6マウス由来胚性幹細胞株。GFP発現細胞株。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0031.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Sugiyama, Fumihiro
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| 樹立者 |
Sugiyama, Fumihiro
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| 寄託日 |
2005
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| 別名 |
RCB2001
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| 動物種 |
_mouse
< Mammals
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| 動物系統名 |
C57BL/6
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| 性別 |
Male
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| 齢 |
embryo
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| 採取組織 |
embryonic stem cells
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| 細胞分類 |
ES
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| 遺伝子改変 |
recombinant
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| 外来遺伝子 |
EGFP
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細胞寿命 |
infinite
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| 細胞形態 |
ES-like
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| Cellosaurus(Expasy) |
CVCL_D275
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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DMEM (high glucose, 1mM Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| 培養容器のコーティング |
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0.1% gelatin coated dish
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播種細胞数 |
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2 x 10 5 cells/60 mm dish
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| 継代・培地交換頻度 |
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Subculture : once/2-3 days
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| フィーダー細胞 |
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MEF (BALB/c)
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| フィーダー細胞の処理方法 |
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X-rays 5000R (or MMC)
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| フィーダー細胞の播種数 |
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3-5 x 10 5 cells/60 mm dish
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
1件
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| Reference(日) |
0件
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| 利用者成果(英) |
15件
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| 利用者成果(日) |
1件
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| 利用者成果(英) |
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11726
Dou X, Zhao Y, Li M, Chen Q, Yamaguchi Y.
Raman imaging diagnosis of the early stage differentiation of mouse embryonic stem cell (mESC).
Spectrochim Acta A Mol Biomol Spectrosc
2020
PubMed ID: 31377684
DOI: 10.1016/j.saa.2019.117438
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11633
Seno A, Murakami C, El-Aarag B, Iwasaki Y, Ohara T, Seno M.
Cancer stem cell induction from mouse embryonic stem cells.
Oncol Lett
2019
PubMed ID: 31452753
DOI: 10.3892/ol.2019.10614
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11862
Afify SM, Chen L, Yan T, Calle AS, Nair N, Murakami C, Zahra MH, Okada N, Iwasaki Y, Seno A, Seno M.
Method to Convert Stem Cells into Cancer Stem Cells.
Methods Protoc
2019
PubMed ID: 31426391
DOI: 10.3390/mps2030071
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17179
Kouno T, Moody J, Kwon AT, Shibayama Y, Kato S, Huang Y, Böttcher M, Motakis E, Mendez M, Severin J, Luginbühl J, Abugessaisa I, Hasegawa A, Takizawa S, Arakawa T, Furuno M, Ramalingam N, West J, Suzuki H, Kasukawa T, Lassmann T, Hon CC, Arner E, Carninci P, Plessy C, Shin JW.
C1 CAGE detects transcription start sites and enhancer activity at single-cell resolution
Nat Commun
2019
10(1):360
PubMed ID: 30664627
DOI: 10.1038/s41467-018-08126-5
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14094
Wilfred Villariza ESPULGAR, Masato SAITO, Jong-Kook LEE, Yoshinori YAMAGUCHI, Eiichi TAMIYA
Non-invasive Video Image-based Analysis Method Coupled to Field Potential Recording for Evaluation of the Drug-induced Effect in Cardiac Tissue
Electrochemistry
2016
84:283-289
DOI: 10.5796/electrochemistry.84.283
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7377
Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Matsumoto T, Nakata K, Mogi M.
Interleukin-1β-induced autophagy-related gene 5 regulates proliferation of embryonic stem cell-derived odontoblastic cells.
PLoS ONE
2015
10:e0124542
PubMed ID: 25894570
DOI: 10.1371/journal.pone.0124542
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15009
Ikeuchi T, Espulgar W, Shimizu E, Saito M, Lee JK, Dou X, Yamaguchi Y, Tamiya E.
Optical microscopy imaging for the diagnosis of the pharmacological reaction of mouse embryonic stem cell-derived cardiomyocytes (mESC-CMs)
Analyst
2015
140(19):6500-7
PubMed ID: 26309911
DOI: 10.1039/c5an01144b
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6195
Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Nakata K, Mogi M.
IL-1β-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway.
Exp Cell Res
2014
328(1):69-86
PubMed ID: 24851717
DOI: 10.1016/j.yexcr.2014.05.006
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9343
N Ozeki, H Yamaguchi, R Kawai, T Hiyama, K Nakata, M Mogi, H Nakamura
Cytokines Induce MMP-3-regulated Proliferation of Embryonic Stem Cell-Derived Odontoblast-Like Cells
Oral Dis
2014
20(5):505-13
PubMed ID: 23902456
DOI: 10.1111/odi.12165
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9346
R Kawai, N Ozeki, H Yamaguchi, T Tanaka, K Nakata, M Mogi, H Nakamura
Mouse ES Cells Have a Potential to Differentiate Into Odontoblast-Like Cells Using Hanging Drop Method
Oral Dis
2014
20(4):395-403
PubMed ID: 23731055
DOI: 10.1111/odi.12134
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15796
Fort A, Hashimoto K, Yamada D, Salimullah M, Keya CA, Saxena A, Bonetti A, Voineagu I, Bertin N, Kratz A, Noro Y, Wong CH, de Hoon M, Andersson R, Sandelin A, Suzuki H, Wei CL, Koseki H, FANTOM Consortium, Hasegawa Y, Forrest AR, Carninci P.
Deep transcriptome profiling of mammalian stem cells supports a regulatory role for retrotransposons in pluripotency maintenance
Nat Genet
2014
46(6):558-66
PubMed ID: 24777452
DOI: 10.1038/ng.2965
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15707
Sathuluri RR, Yoshikawa H, Shimizu E, Saito M, Tamiya E.
Gold nanoparticle-based surface-enhanced Raman scattering for noninvasive molecular probing of embryonic stem cell differentiation
PLoS One
2011
6(8):e22802
PubMed ID: 21829653
DOI: 10.1371/journal.pone.0022802
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8095
Ishikawa K, Toyama-Sorimachi N, Nakada K, Morimoto M, Imanishi H, Yoshizaki M, Sasawatari S, Niikura M, Takenaga K, Yonekawa H, Hayashi J.
The innate immune system in host mice targets cells with allogenic mitochondrial DNA.
J. Exp. Med.
2010
207:2297-305
PubMed ID: 20937705
DOI: 10.1084/jem.20092296
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8243
Hossain MM, Shimizu E, Saito M, Rao SR, Yamaguchi Y, Tamiya E.
Non-invasive characterization of mouse embryonic stem cell derived cardiomyocytes based on the intensity variation in digital beating video.
Analyst
2010
135:1624-30
PubMed ID: 20517541
DOI: 10.1039/c0an00208a
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8409
Hayashi Y, Furue MK, Tanaka S, Hirose M, Wakisaka N, Danno H, Ohnuma K, Oeda S, Aihara Y, Shiota K, Ogura A, Ishiura S, Asashima M.
BMP4 induction of trophoblast from mouse embryonic stem cells in defined culture conditions on laminin.
In Vitro Cell. Dev. Biol. Anim.
2010
46:416-30
PubMed ID: 20033790
DOI: 10.1007/s11626-009-9266-6
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