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Cell No. : Cell Name
AES0003 : B6G-2  update : 2023/03/28
CommentEmbryonic stem (ES) cell line derived from C57BL/6 mouse. Expressing GFP.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sugiyama, Fumihiro
Originator Sugiyama, Fumihiro
Year of deposit 2005
Another name RCB2001
Animal mouse < Mammals
Strain name C57BL/6
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene EGFP
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_D275
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, 1mM Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF (BALB/c)
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10^(5) cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 15

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3092  Shimizukawa, Rie, Sakata, Aya, Hirose, Michiko, Takahashi, Akio, Iseki, Hiroyoshi, Liu, Ying, Kunita, Satoshi, Sugiyama, Fumihiro, Yagami, Ken-ichi  Establishment of a new embryonic stem cell line derived from C57BL/6 mouse expressing EGFP ubiquitously.  Genesis  2005  42:47-52  PubMed ID: 15830376   DOI: 10.1002/gene.20122

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User's Publication
11726  Dou X, Zhao Y, Li M, Chen Q, Yamaguchi Y.  Raman imaging diagnosis of the early stage differentiation of mouse embryonic stem cell (mESC).  Spectrochim Acta A Mol Biomol Spectrosc  2020    PubMed ID: 31377684   DOI: 10.1016/j.saa.2019.117438
11633  Seno A, Murakami C, El-Aarag B, Iwasaki Y, Ohara T, Seno M.  Cancer stem cell induction from mouse embryonic stem cells.  Oncol Lett  2019    PubMed ID: 31452753   DOI: 10.3892/ol.2019.10614
11862  Afify SM, Chen L, Yan T, Calle AS, Nair N, Murakami C, Zahra MH, Okada N, Iwasaki Y, Seno A, Seno M.  Method to Convert Stem Cells into Cancer Stem Cells.  Methods Protoc  2019    PubMed ID: 31426391   DOI: 10.3390/mps2030071
17179  Kouno T, Moody J, Kwon AT, Shibayama Y, Kato S, Huang Y, Böttcher M, Motakis E, Mendez M, Severin J, Luginbühl J, Abugessaisa I, Hasegawa A, Takizawa S, Arakawa T, Furuno M, Ramalingam N, West J, Suzuki H, Kasukawa T, Lassmann T, Hon CC, Arner E, Carninci P, Plessy C, Shin JW.  C1 CAGE detects transcription start sites and enhancer activity at single-cell resolution  Nat Commun  2019  10(1):360  PubMed ID: 30664627   DOI: 10.1038/s41467-018-08126-5
14094  Wilfred Villariza ESPULGAR, Masato SAITO, Jong-Kook LEE, Yoshinori YAMAGUCHI, Eiichi TAMIYA  Non-invasive Video Image-based Analysis Method Coupled to Field Potential Recording for Evaluation of the Drug-induced Effect in Cardiac Tissue  Electrochemistry  2016  84:283-289    DOI: 10.5796/electrochemistry.84.283
7377  Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Matsumoto T, Nakata K, Mogi M.  Interleukin-1β-induced autophagy-related gene 5 regulates proliferation of embryonic stem cell-derived odontoblastic cells.  PLoS ONE  2015  10:e0124542  PubMed ID: 25894570   DOI: 10.1371/journal.pone.0124542
15009  Ikeuchi T, Espulgar W, Shimizu E, Saito M, Lee JK, Dou X, Yamaguchi Y, Tamiya E.  Optical microscopy imaging for the diagnosis of the pharmacological reaction of mouse embryonic stem cell-derived cardiomyocytes (mESC-CMs)  Analyst  2015  140(19):6500-7  PubMed ID: 26309911   DOI: 10.1039/c5an01144b
6195  Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Nakata K, Mogi M.  IL-1β-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway.  Exp Cell Res  2014  328(1):69-86  PubMed ID: 24851717   DOI: 10.1016/j.yexcr.2014.05.006
9343  N Ozeki, H Yamaguchi, R Kawai, T Hiyama, K Nakata, M Mogi, H Nakamura  Cytokines Induce MMP-3-regulated Proliferation of Embryonic Stem Cell-Derived Odontoblast-Like Cells  Oral Dis  2014  20(5):505-13  PubMed ID: 23902456   DOI: 10.1111/odi.12165
9346  R Kawai, N Ozeki, H Yamaguchi, T Tanaka, K Nakata, M Mogi, H Nakamura  Mouse ES Cells Have a Potential to Differentiate Into Odontoblast-Like Cells Using Hanging Drop Method  Oral Dis  2014  20(4):395-403  PubMed ID: 23731055   DOI: 10.1111/odi.12134
15796  Fort A, Hashimoto K, Yamada D, Salimullah M, Keya CA, Saxena A, Bonetti A, Voineagu I, Bertin N, Kratz A, Noro Y, Wong CH, de Hoon M, Andersson R, Sandelin A, Suzuki H, Wei CL, Koseki H, FANTOM Consortium, Hasegawa Y, Forrest AR, Carninci P.  Deep transcriptome profiling of mammalian stem cells supports a regulatory role for retrotransposons in pluripotency maintenance  Nat Genet  2014  46(6):558-66  PubMed ID: 24777452   DOI: 10.1038/ng.2965
15707  Sathuluri RR, Yoshikawa H, Shimizu E, Saito M, Tamiya E.  Gold nanoparticle-based surface-enhanced Raman scattering for noninvasive molecular probing of embryonic stem cell differentiation  PLoS One  2011  6(8):e22802  PubMed ID: 21829653   DOI: 10.1371/journal.pone.0022802
8095  Ishikawa K, Toyama-Sorimachi N, Nakada K, Morimoto M, Imanishi H, Yoshizaki M, Sasawatari S, Niikura M, Takenaga K, Yonekawa H, Hayashi J.  The innate immune system in host mice targets cells with allogenic mitochondrial DNA.  J. Exp. Med.  2010  207:2297-305  PubMed ID: 20937705   DOI: 10.1084/jem.20092296
8243  Hossain MM, Shimizu E, Saito M, Rao SR, Yamaguchi Y, Tamiya E.  Non-invasive characterization of mouse embryonic stem cell derived cardiomyocytes based on the intensity variation in digital beating video.  Analyst  2010  135:1624-30  PubMed ID: 20517541   DOI: 10.1039/c0an00208a
8409  Hayashi Y, Furue MK, Tanaka S, Hirose M, Wakisaka N, Danno H, Ohnuma K, Oeda S, Aihara Y, Shiota K, Ogura A, Ishiura S, Asashima M.  BMP4 induction of trophoblast from mouse embryonic stem cells in defined culture conditions on laminin.  In Vitro Cell. Dev. Biol. Anim.  2010  46:416-30  PubMed ID: 20033790   DOI: 10.1007/s11626-009-9266-6

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