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Cell No. : Cell Name
AES0003 : B6G-2  update : 2018/07/06
CommentEmbryonic stem (ES) cell line derived from C57BL/6 mouse. Expressing GFP.
Comment from the depositor
Terms and conditions1) Basically, there is no restriction regarding academic use. 2) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sugiyama, Fumihiro
Originator Sugiyama, Fumihiro
Year of deposit 2005
Another name RCB2001
Animal _mouse < Mammals
Strain name C57BL/6
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene EGFP
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, 1mM Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF (BALB/c)
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10^(5) cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 10

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3092  Shimizukawa, Rie, Sakata, Aya, Hirose, Michiko, Takahashi, Akio, Iseki, Hiroyoshi, Liu, Ying, Kunita, Satoshi, Sugiyama, Fumihiro, Yagami, Ken-ichi  Establishment of a new embryonic stem cell line derived from C57BL/6 mouse expressing EGFP ubiquitously.  Genesis  2005  42:47-52  PubMed ID: 15830376

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User's Publication
11726  Dou X, Zhao Y, Li M, Chen Q, Yamaguchi Y.  Raman imaging diagnosis of the early stage differentiation of mouse embryonic stem cell (mESC).  Spectrochim Acta A Mol Biomol Spectrosc  2020    PubMed ID: 31377684
11633  Seno A, Murakami C, El-Aarag B, Iwasaki Y, Ohara T, Seno M.  Cancer stem cell induction from mouse embryonic stem cells.  Oncol Lett  2019    PubMed ID: 31452753
11862  Afify SM, Chen L, Yan T, Calle AS, Nair N, Murakami C, Zahra MH, Okada N, Iwasaki Y, Seno A, Seno M.  Method to Convert Stem Cells into Cancer Stem Cells.  Methods Protoc  2019    PubMed ID: 31426391
7377  Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Matsumoto T, Nakata K, Mogi M.  Interleukin-1β-induced autophagy-related gene 5 regulates proliferation of embryonic stem cell-derived odontoblastic cells.  PLoS ONE  2015  10:e0124542  PubMed ID: 25894570
6195  Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Nakata K, Mogi M.  IL-1β-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway.  Exp Cell Res  2014  328(1):69-86  PubMed ID: 24851717
9343  N Ozeki, H Yamaguchi, R Kawai, T Hiyama, K Nakata, M Mogi, H Nakamura  Cytokines Induce MMP-3-regulated Proliferation of Embryonic Stem Cell-Derived Odontoblast-Like Cells  Oral Dis  2014  20(5):505-13  PubMed ID: 23902456
9346  R Kawai, N Ozeki, H Yamaguchi, T Tanaka, K Nakata, M Mogi, H Nakamura  Mouse ES Cells Have a Potential to Differentiate Into Odontoblast-Like Cells Using Hanging Drop Method  Oral Dis  2014  20(4):395-403  PubMed ID: 23731055
8095  Ishikawa K, Toyama-Sorimachi N, Nakada K, Morimoto M, Imanishi H, Yoshizaki M, Sasawatari S, Niikura M, Takenaga K, Yonekawa H, Hayashi J.  The innate immune system in host mice targets cells with allogenic mitochondrial DNA.  J. Exp. Med.  2010  207:2297-305  PubMed ID: 20937705
8243  Hossain MM, Shimizu E, Saito M, Rao SR, Yamaguchi Y, Tamiya E.  Non-invasive characterization of mouse embryonic stem cell derived cardiomyocytes based on the intensity variation in digital beating video.  Analyst  2010  135:1624-30  PubMed ID: 20517541
8409  Hayashi Y, Furue MK, Tanaka S, Hirose M, Wakisaka N, Danno H, Ohnuma K, Oeda S, Aihara Y, Shiota K, Ogura A, Ishiura S, Asashima M.  BMP4 induction of trophoblast from mouse embryonic stem cells in defined culture conditions on laminin.  In Vitro Cell. Dev. Biol. Anim.  2010  46:416-30  PubMed ID: 20033790

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