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Cell No. : Cell Name
AES0002 : GS-DG1  update : 2024/07/09
CommentGermline stem cell line derived from mouse testis. Protocol
Comment from the depositor
Terms and conditions1) A permission of the approver (Dr.Shinohara, Takashi or Dr.Shinohara, Mito) is required. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biol Reprod 2003;69:612-616) designated by the DEPOSITOR is required. 3) The BIOLOGICAL RESOURCE should not be used for commercial purposes.
approver's address
Department of Molecular Genetics
Kyoto University Graduate School of Medicine
53 ShogoinKawaramachi, Sakyo-ku, Kyoto 606-8507, Japan
Fax. +81-75-751-4169

Department of Molecular Genetics
Kyoto University Graduate School of Medicine
53 ShogoinKawaramachi, Sakyo-ku, Kyoto 606-8507, JAPAN
Fax. +81-75-751-4169
Order Form Order Form(C-0032.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Shinohara, Takashi
Originator Shinohara, Takashi
Year of deposit 2004
Another name RCB1968
Animal _mouse < Mammals
Strain name DBA/2
Gender Male
Age at sampling 1 day
Tissue testis
Classification ES
Year of origin 2003
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_E268
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium Medium and additives(excel:20KB)
Antibiotics Free
Passage method 0.25% Trypsin, add Iscove's + 5mg/ml BSA to stop the reaction
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2.5 x 10 5 cells/well/6 well plate
SC frequency Subculture : once/5 days, Medium Renewal : once/2-3days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10 5 cells/well/6 well plate
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 3

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3257  Kanatsu-Shinohara, Mito, Ogonuki, Narumi, Inoue, Kimiko, Miki, Hiromi, Ogura, Atsuo, Toyokuni, Shinya, Shinohara, Takashi  Long-term proliferation in culture and germline transmission of mouse male germline stem cells.  Biol Reprod  2003  69:612-6  PubMed ID: 12700182   DOI: 10.1095/biolreprod.103.017012

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User's Publication
11259  Sato T, Ogawa T.  Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method.  Methods Mol. Biol.  2019    PubMed ID: 30353509   DOI: 10.1007/978-1-4939-8831-0_5
18462  Shiura H, Ikeda R, Lee J, Sato T, Ogonuki N, Hirose M, Ogura A, Ogawa T, Abe K.  Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse  Genesis  2013  51(7):498-505  PubMed ID: 23529902   DOI: 10.1002/dvg.22391
16083  Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.  Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome  DNA Res  2013  20(6):549-65  PubMed ID: 23861320   DOI: 10.1093/dnares/dst030

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