細胞番号 : 細胞名
AES0001 : H-1
update : 2023/04/11
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| 細胞特性(Comment:英) | In vitro differentiation to various type tissue cells(e.g. neuron,cardiomyocyte,hematopoietic cells) |
| 細胞特性(日) | 試験管内分化誘導実験にて、神経系細胞、心筋細胞、血液系細胞などへの分化が確認されている。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0031.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Atsumi, Tadao
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| 樹立者 |
Kitani, Hiroshi
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| 寄託日 |
2001
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| 別名 |
RCB1778
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| 動物種 |
_mouse
< Mammals
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| 動物系統名 |
C3H
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| 性別 |
Male
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| 齢 |
embryo
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| 採取組織 |
embryonic stem cells
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| 細胞分類 |
ES
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細胞寿命 |
infinite
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| 細胞形態 |
ES-like
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| Cellosaurus(Expasy) |
CVCL_N541
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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DMEM (low glucose) + 15% FBS + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| 培養容器のコーティング |
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0.1% gelatin coated dish
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播種細胞数 |
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5 x 10 5 cells/60 mm dish
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| 継代・培地交換頻度 |
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Subculture or Medium Renewal : once/2-3 days
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| フィーダー細胞 |
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C57BL/6J-emb
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| フィーダー細胞 番号 |
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RCB1312
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| フィーダー細胞の処理方法 |
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X-rays 5000R (or MMC)
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| フィーダー細胞の播種数 |
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1-2 x 10 5 cells/60 mm dish
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
4件
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| Reference(日) |
0件
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| 利用者成果(英) |
15件
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| 利用者成果(日) |
0件
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| Reference(英) |
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2678
Anzai H, Ikawa Y, Atsumi T
Stem cell factor and interleukin-3 induce stepwise generation of erythroid precursor cells from a basic fibroblast growth factor-dependent hematopoietic stem cell line, A-6.
Biochem Biophys Res Commun
2001
282:940-6
PubMed ID: 11352642
DOI: 10.1006/bbrc.2001.4674
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2564
Anzai H, Nagayoshi M, Obata M, Ikawa Y, Atsumi T
Self-renewal and differentiation of a basic fibroblast growth factor-dependent multipotent hematopoietic cell line derived from embryonic stem cells.
Dev Growth Differ
1999
41:51-8
PubMed ID: 10445502
DOI: 10.1046/j.1440-169x.1999.00412.x
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2562
Kitani H, Takagi N, Atsumi T, Kawakura K, Imamura K, Goto S, Kusakabe M, Fukuta K
Isolation of a germline-transmissible embryonic stem (ES) cell line from C3H/He mice.
Zoolog sci
1996
13:865-871
PubMed ID: 9107142
DOI: 10.2108/zsj.13.865
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2563
Atsumi, T. et al
Dev Growth Differ
1995
37:167-172
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| 利用者成果(英) |
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9809
Hidenori Tani, Taro Matsutani, Hiroshi Aoki, Kaoru Nakamura, Yu Hamaguchi, Tetsuya Nakazato, Michiaki Hamada
Identification of RNA Biomarkers for Chemical Safety Screening in Neural Cells Derived From Mouse Embryonic Stem Cells Using RNA Deep Sequencing Analysis
Biochem Biophys Res Commun
2019
512(4):641-646
PubMed ID: 30497775
DOI: 10.1016/j.bbrc.2018.11.141
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10612
Tani H, Takeshita JI, Aoki H, Nakamura K, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Torimura M, Sato H.
Effect of methyl p-hydroxybenzoate on the culture of mammalian cell.
Drug Discov Ther
2017
11:276-280
PubMed ID: 29162775
DOI: 10.5582/ddt.2017.01054
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14691
Tani H, Takeshita JI, Aoki H, Nakamura K, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Sato H, Torimura M.
Identification of RNA biomarkers for chemical safety screening in mouse embry
PLoS One
2017
12(7):e0182032
PubMed ID: 28750099
DOI: 10.1371/journal.pone.0182032
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9608
Tani H, Takeshita J, Aoki H, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Torimura M.
Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene.
J. Biosci. Bioeng.
2016
122:329-33
PubMed ID: 26975756
DOI: 10.1016/j.jbiosc.2016.02.007
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15736
Fujikawa-Yamamoto K, Ota T, Miyagoshi M, Yamagishi H.
Pluripotency of a polyploid H1 (ES) cell system without leukaemia inhibitory factor
Cell Prolif
2012
45(2):140-7
PubMed ID: 22288737
DOI: 10.1111/j.1365-2184.2011.00805.x
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7816
Fujikawa-Yamamoto K, Miyagoshi M, Ota T, Yamagishi H.
Haploid unit-ploidy transition of tetraploid and octaploid H1 (ES) cells in long-term culturing.
Hum. Cell
2011
24:78-85
PubMed ID: 21573878
DOI: 10.1007/s13577-011-0017-0
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7828
Fujikawa-Yamamoto K, Miyagoshi M, Luo X, Yamagishi H.
Hexaploid H1 (ES) cells established from octaploid H1 cells are as DNA stable as pentaploid H1 cells.
Hum. Cell
2011
24:13-20
PubMed ID: 21547691
DOI: 10.1007/s13577-010-0003-y
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7882
Fujikawa-Yamamoto K, Miyagoshi M, Luo X, Yamagishi H.
DNA-unstable decaploid mouse H1 (ES) cells established from DNA-stable pentaploid H1 (ES) cells polyploidized using demecolcine.
Cell Prolif.
2011
44:111-9
PubMed ID: 21401752
DOI: 10.1111/j.1365-2184.2011.00734.x
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19152
Masanobu Horie, Akira Ito
A Genetically Engineered STO Feeder System Expressing E-Cadherin and Leukemia Inhibitory Factor for Mouse Pluripotent Stem Cell Culture
Journal of Bioprocessing & Biotechniques
2011
01
DOI: 10.4172/2155-9821.s3-001
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8026
Luo X, Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.
Different responses between diploid and tetraploid H1 embryonic stem cells appeared during long-term culturing in L15F10 medium without leukemia inhibitory factor.
Hum. Cell
2010
23:134-40
PubMed ID: 21166884
DOI: 10.1111/j.1749-0774.2010.00094.x
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8215
Horie M, Ito A, Kiyohara T, Kawabe Y, Kamihira M.
E-cadherin gene-engineered feeder systems for supporting undifferentiated growth of mouse embryonic stem cells.
J. Biosci. Bioeng.
2010
110:582-7
PubMed ID: 20587371
DOI: 10.1016/j.jbiosc.2010.06.002
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14693
Sakai S, Hashimoto I, Kawakami K.
Production of cell-enclosing hollow-core agarose microcapsules via jetting in water-immiscible liquid paraffin and formation of embryoid body-like spherical tissues from mouse ES cells enclosed within these microcapsules
Biotechnol Bioeng
2008
99(1):235-43
PubMed ID: 17705234
DOI: 10.1002/bit.21624
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14694
Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H, Luo X.
Alteration and preservation of cellular characteristics in long-term culture of tetraploid H-1 (ES) cells
Hum Cell
2008
21(2):18-27
PubMed ID: 18397471
DOI: 10.1111/j.1749-0774.2008.00047.x
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14692
Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.
Establishment of a tetraploid cell line from mouse H-1 (ES) cells highly polyploidized with demecolcine
Cell Prolif
2007
40(3):327-37
PubMed ID: 17531078
DOI: 10.1111/j.1365-2184.2007.00442.x
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8912
Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.
Polyploidization of mouse H-1 (ES) cells by demecolcine and K-252a .
Cytologia
2006
71:399-406
DOI: 10.1508/cytologia.71.399
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