Cell No. : Cell Name
AES0001 : H-1
update : 2023/04/11
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| Comment | In vitro differentiation to various type tissue cells(e.g. neuron,cardiomyocyte,hematopoietic cells) |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Atsumi, Tadao
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| Originator |
Kitani, Hiroshi
|
| Year of deposit |
2001
|
| Another name |
RCB1778
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
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| Classification |
ES
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_N541
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (low glucose) + 15% FBS + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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5 x 10 5 cells/60 mm dish
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| SC frequency |
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Subculture or Medium Renewal : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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C57BL/6J-emb
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| Feeder cells No. |
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RCB1312
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1-2 x 10 5 cells/60 mm dish
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
15
|
| Reference |
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2678
Anzai H, Ikawa Y, Atsumi T
Stem cell factor and interleukin-3 induce stepwise generation of erythroid precursor cells from a basic fibroblast growth factor-dependent hematopoietic stem cell line, A-6.
Biochem Biophys Res Commun
2001
282:940-6
PubMed ID: 11352642
DOI: 10.1006/bbrc.2001.4674
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2564
Anzai H, Nagayoshi M, Obata M, Ikawa Y, Atsumi T
Self-renewal and differentiation of a basic fibroblast growth factor-dependent multipotent hematopoietic cell line derived from embryonic stem cells.
Dev Growth Differ
1999
41:51-8
PubMed ID: 10445502
DOI: 10.1046/j.1440-169x.1999.00412.x
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2562
Kitani H, Takagi N, Atsumi T, Kawakura K, Imamura K, Goto S, Kusakabe M, Fukuta K
Isolation of a germline-transmissible embryonic stem (ES) cell line from C3H/He mice.
Zoolog sci
1996
13:865-871
PubMed ID: 9107142
DOI: 10.2108/zsj.13.865
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2563
Atsumi, T. et al
Dev Growth Differ
1995
37:167-172
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| User's Publication |
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9809
Hidenori Tani, Taro Matsutani, Hiroshi Aoki, Kaoru Nakamura, Yu Hamaguchi, Tetsuya Nakazato, Michiaki Hamada
Identification of RNA Biomarkers for Chemical Safety Screening in Neural Cells Derived From Mouse Embryonic Stem Cells Using RNA Deep Sequencing Analysis
Biochem Biophys Res Commun
2019
512(4):641-646
PubMed ID: 30497775
DOI: 10.1016/j.bbrc.2018.11.141
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10612
Tani H, Takeshita JI, Aoki H, Nakamura K, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Torimura M, Sato H.
Effect of methyl p-hydroxybenzoate on the culture of mammalian cell.
Drug Discov Ther
2017
11:276-280
PubMed ID: 29162775
DOI: 10.5582/ddt.2017.01054
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14691
Tani H, Takeshita JI, Aoki H, Nakamura K, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Sato H, Torimura M.
Identification of RNA biomarkers for chemical safety screening in mouse embry
PLoS One
2017
12(7):e0182032
PubMed ID: 28750099
DOI: 10.1371/journal.pone.0182032
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9608
Tani H, Takeshita J, Aoki H, Abe R, Toyoda A, Endo Y, Miyamoto S, Gamo M, Torimura M.
Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene.
J. Biosci. Bioeng.
2016
122:329-33
PubMed ID: 26975756
DOI: 10.1016/j.jbiosc.2016.02.007
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15736
Fujikawa-Yamamoto K, Ota T, Miyagoshi M, Yamagishi H.
Pluripotency of a polyploid H1 (ES) cell system without leukaemia inhibitory factor
Cell Prolif
2012
45(2):140-7
PubMed ID: 22288737
DOI: 10.1111/j.1365-2184.2011.00805.x
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7816
Fujikawa-Yamamoto K, Miyagoshi M, Ota T, Yamagishi H.
Haploid unit-ploidy transition of tetraploid and octaploid H1 (ES) cells in long-term culturing.
Hum. Cell
2011
24:78-85
PubMed ID: 21573878
DOI: 10.1007/s13577-011-0017-0
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7828
Fujikawa-Yamamoto K, Miyagoshi M, Luo X, Yamagishi H.
Hexaploid H1 (ES) cells established from octaploid H1 cells are as DNA stable as pentaploid H1 cells.
Hum. Cell
2011
24:13-20
PubMed ID: 21547691
DOI: 10.1007/s13577-010-0003-y
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7882
Fujikawa-Yamamoto K, Miyagoshi M, Luo X, Yamagishi H.
DNA-unstable decaploid mouse H1 (ES) cells established from DNA-stable pentaploid H1 (ES) cells polyploidized using demecolcine.
Cell Prolif.
2011
44:111-9
PubMed ID: 21401752
DOI: 10.1111/j.1365-2184.2011.00734.x
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19152
Masanobu Horie, Akira Ito
A Genetically Engineered STO Feeder System Expressing E-Cadherin and Leukemia Inhibitory Factor for Mouse Pluripotent Stem Cell Culture
Journal of Bioprocessing & Biotechniques
2011
01
DOI: 10.4172/2155-9821.s3-001
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8026
Luo X, Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.
Different responses between diploid and tetraploid H1 embryonic stem cells appeared during long-term culturing in L15F10 medium without leukemia inhibitory factor.
Hum. Cell
2010
23:134-40
PubMed ID: 21166884
DOI: 10.1111/j.1749-0774.2010.00094.x
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8215
Horie M, Ito A, Kiyohara T, Kawabe Y, Kamihira M.
E-cadherin gene-engineered feeder systems for supporting undifferentiated growth of mouse embryonic stem cells.
J. Biosci. Bioeng.
2010
110:582-7
PubMed ID: 20587371
DOI: 10.1016/j.jbiosc.2010.06.002
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14693
Sakai S, Hashimoto I, Kawakami K.
Production of cell-enclosing hollow-core agarose microcapsules via jetting in water-immiscible liquid paraffin and formation of embryoid body-like spherical tissues from mouse ES cells enclosed within these microcapsules
Biotechnol Bioeng
2008
99(1):235-43
PubMed ID: 17705234
DOI: 10.1002/bit.21624
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14694
Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H, Luo X.
Alteration and preservation of cellular characteristics in long-term culture of tetraploid H-1 (ES) cells
Hum Cell
2008
21(2):18-27
PubMed ID: 18397471
DOI: 10.1111/j.1749-0774.2008.00047.x
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14692
Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.
Establishment of a tetraploid cell line from mouse H-1 (ES) cells highly polyploidized with demecolcine
Cell Prolif
2007
40(3):327-37
PubMed ID: 17531078
DOI: 10.1111/j.1365-2184.2007.00442.x
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8912
Fujikawa-Yamamoto K, Miyagoshi M, Yamagishi H.
Polyploidization of mouse H-1 (ES) cells by demecolcine and K-252a .
Cytologia
2006
71:399-406
DOI: 10.1508/cytologia.71.399
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