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Cell No. : Cell Name
RCB5683 : ASPS-KY  update : 2024/01/17
Comment
Comment from the depositorHuman cell line derived from alveolar soft part sarcoma.
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Expected time Upon a request we will start preparation. Contact us: cellkitaku.brc@riken.jp
Depositor MIYAGI, Youhei
Originator KUSHIDA, Kazuyoshi & YANOMA, Shunsuke
Animal _human < Mammals
Strain name Homo
Genus sapiens
Gender Female
Age at sampling 27 years
Tissue lung meta
Primary focus lower leg
Disease name alveolar soft part sarcoma
Metastatic ability Yes
Metastatic tissue lumg
Classification cancer
Lifespan infinite
Morphology epithelial-like
Product HGF, IL-8
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium RPMI1640 + 10% FCS RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1: 3-4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus OK
Reference information Reference 11
User's Publication 0


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Reference
16681  Mukaihara K, Tanabe Y, Kubota D, Akaike K, Hayashi T, Mogushi K, Hosoya M, Sato S, Kobayashi E, Okubo T, Kim Y, Kohsaka S, Saito T, Kaneko K, Suehara Y.  Cabozantinib and dastinib exert anti-tumor activity in alveolar soft part sarcoma  PLoS One  2017  12(9):e0185321  PubMed ID: 28945796   DOI: 10.1371/journal.pone.0185321
16674  Kim Y, Kobayashi E, Kubota D, Suehara Y, Mukaihara K, Akaike K, Ito A, Kaneko K, Chuman H, Kawai A, Kitano S.  Reduced argininosuccinate synthetase expression in refractory sarcomas: Impacts on therapeutic potential and drug resistance  Oncotarget  2016  7(43):70832-70844  PubMed ID: 27683125   DOI: 10.18632/oncotarget.12225
16682  Nakayama R, Zhang YX, Czaplinski JT, Anatone AJ, Sicinska ET, Fletcher JA, Demetri GD, Wagner AJ.  Preclinical activity of selinexor, an inhibitor of XPO1, in sarcoma  Oncotarget  2016  7(13):16581-92  PubMed ID: 26918731   DOI: 10.18632/oncotarget.7667
16680  Kusabe Y, Kawashima H, Ogose A, Sasaki T, Ariizumi T, Hotta T, Endo N.  Effect of temozolomide on the viability of musculoskeletal sarcoma cells  Oncol Lett  2015  10(4):2511-2518  PubMed ID: 26622881   DOI: 10.3892/ol.2015.3506
16684  Kubota D, Yoshida A, Kawai A, Kondo T.  Proteomics identified overexpression of SET oncogene product and possible therapeutic utility of protein phosphatase 2A in alveolar soft part sarcoma  J Proteome Res  2014  13(5):2250-61  PubMed ID: 24621013   DOI: 10.1021/pr400929h
16683  Li GD, Kawashima H, Ogose A, Ariizumi T, Hotta T, Kuwano R, Urata Y, Fujiwara T, Endo N.  Telomelysin shows potent antitumor activity through apoptotic and non-apoptotic cell death in soft tissue sarcoma cells  Cancer Sci  2013  104(9):1178-88  PubMed ID: 23718223   DOI: 10.1111/cas.12208
16676  Xu Y, Ogose A, Kawashima H, Hotta T, Ariizumi T, Li G, Umezu H, Endo N.  High-level expression of podoplanin in benign and malignant soft tissue tumors: immunohistochemical and quantitative real-time RT-PCR analysis  Oncol Rep  2011  25(3):599-60  PubMed ID: 21234520   DOI: 10.3892/or.2011.1141
16679  Li G, Kawashima H, Ji L, Ogose A, Ariizumi T, Umezu H, Xu Y, Hotta T, Endo N.  Frequent absence of tumor suppressor FUS1 protein expression in human bone and soft tissue sarcomas  Anticancer Res  2011  31(1):11-21  PubMed ID: 21273575  
16685  Hoshino M, Ogose A, Kawashima H, Izumi T, Hotta T, Hatano H, Morita T, Otsuka H, Umezu H, Yanoma S, Tsukuda M, Endo N.  Molecular analyses of cell origin and detection of circulating tumor cells in the peripheral blood in alveolar soft part sarcoma  Cancer Genet Cytogenet  2009  190(2):75-80  PubMed ID: 19380023   DOI: 10.1016/j.cancergencyto.2008.11.014
16677  Okamoto Y, Ozaki T, Miyazaki K, Aoyama M, Miyazaki M, Nakagawara A.  UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme  Cancer Res  2003  63(14):4167-73  PubMed ID: 12874022  
16675  Hanaoka E, Ozaki T, Nakamura Y, Moriya H, Nakagawara A, Sakiyama S.  Overexpression of DAN causes a growth suppression in p53-deficient SAOS-2 cells  Biochem Biophys Res Commun  2000  278(1):20-6  PubMed ID: 11071849   DOI: 10.1006/bbrc.2000.3758

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