細胞番号 : 細胞名
RCB5661 : HPL1A
update : 2024/02/07
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| 細胞特性(Comment:英) | RCB5661 HPL1A、RCB5662 HPL1D were derived from the same patient. |
| 細胞特性(日) | RCB5661 HPL1A、RCB5662 HPL1Dは同一人由来。 |
| 細胞特性(寄託者記述:英) | Immortalized human peripheral lung epithelial cell line.The HPL1A and HPL1D lines were established by transfecting the SV40 large T antigen gene from the normal lung of a 74-year-old female donor, who had never smoked and underwent surgery for a small well-demarcated squamous cell carcinoma of the earliest disease stage (pT1N0M0, stage I). Precancerous regions of peripheral lung such as atypical adenomatous hyperplasia were not noted in the lung specimen by the conventional pathological examination. Considering the uniform integration pattern observed for the SV40 large T antigen gene, the HPL1A and HPL1D lines appear to have arisen from a single immortalized cell. |
| 細胞特性(寄託者記述:日) | ヒト正常肺由来不死化末梢気道上皮細胞株。 |
| 使用条件(英) | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| 使用条件(日) | 研究成果の公表にあたって謝辞の表明を必要とする。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
TAKAHASHI, Takashi
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| 樹立者 |
TAKAHASHI, Takashi
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| 寄託日 |
2022
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| クローニング（寄託者） |
Yes
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| クローニングの方法（寄託者） |
cloning ring method
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 性別 |
Female
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| 齢 |
74 years
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| 原発組織 |
normal peripheral lung specimens
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| 細胞分類 |
other
(immortalized)
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| 遺伝子改変 |
recombinant
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| 外来遺伝子 |
SV40 large T antigen gene
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細胞寿命 |
infinite
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| 細胞形態 |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_9863
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
Adherent cells
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Adherent cells
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| 培地 |
F12 (Sigma-Aldrich #N6658) + 1% FBS + 5μg/ml bovine insulin + 5μg/ml human transferrin + 10 -7M hydrocortisone + 2x10 -10M triiodo thyronine
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HamF12 + 1% FBS + 5μg/ml bovine insulin + 5μg/ml human transferrin + 10 -7M hydrocortisone + 2x10 -10M triiodo thyronine
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin
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播種細胞数 |
3 x 10 5cells/ 10 cm dish
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3-6 x 10 5 cells/100 mm dish
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| 継代密度 |
1 : 5 split
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| 継代・培地交換頻度 |
every 3 days
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
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| 培養最適温度 |
37
℃
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37
℃
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| 二酸化炭素濃度 |
5
%
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5
%
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| 凍結培地 |
CELLBANKER (ZENOGEN PHARMA #DS-CB011)
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CELLBANKER 1
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| 動物種PCR |
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OK
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| 個体識別検査 |
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OK
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| 文献情報 |
Reference(英) |
12件
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| Reference(日) |
0件
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| 利用者成果(英) |
0件
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| 利用者成果(日) |
0件
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| Reference(英) |
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16254
Vu T, Yang S, Datta PK.
MiR-216b/Smad3/BCL-2 Axis Is Involved in Smoking-Mediated Drug Resistance in Non-Small Cell Lung Cancer
Cancers (Basel)
2020
12(7):1879
PubMed ID: 32668597
DOI: 10.3390/cancers12071879
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16253
Almosnid NM, Gao Y, He C, Park HS, Altman E.
In vitro antitumor effects of two novel oligostilbenes, cis- and trans-suffruticosol D, isolated from Paeonia suffruticosa seeds
Int J Oncol
2016
48(2):646-56.
PubMed ID: 26647827
DOI: 10.3892/ijo.2015.3269
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16252
Gao Y, He C, Ran R, Zhang D, Li D, Xiao PG, Altman E.
The resveratrol oligomers, cis- and trans-gnetin H, from Paeonia suffruticosa seeds inhibit the growth of several human cancer cell lines
J Ethnopharmacol
2015
169:24-33
PubMed ID: 25862967
DOI: 10.1016/j.jep.2015.03.074
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16251
Roth FC, Mulder JE, Brien JF, Takahashi T, Massey TE.
Cytotoxic interaction between amiodarone and desethylamiodarone in human peripheral lung epithelial cells
Chem Biol Interact
2013
204(3):135-9
PubMed ID: 23707191
DOI: 10.1016/j.cbi.2013.05.006
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16249
Samanta D, Gonzalez AL, Nagathihalli N, Ye F, Carbone DP, Datta PK.
Smoking attenuates transforming growth factor-β-mediated tumor suppression function through downregulation of Smad3 in lung cancer
Cancer Prev Res (Phila)
2012
5(3):453-63
PubMed ID: 22232600
DOI: 10.1158/1940-6207.CAPR-11-0313
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16250
Samanta D, Kaufman J, Carbone DP, Datta PK.
Long-term smoking mediated down-regulation of Smad3 induces resistance to carboplatin in non-small cell lung cancer
Neoplasia
2012
14(7):644-55
PubMed ID: 22904681
DOI: 10.1593/neo.12548
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16248
Mulder JE, Brien JF, Racz WJ, Takahashi T, Massey TE.
Mechanisms of amiodarone and desethylamiodarone cytotoxicity in nontransformed human peripheral lung epithelial cells
J Pharmacol Exp Ther
2011
336(2):551-9
PubMed ID: 21078785
DOI: 10.1124/jpet.110.173120
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16247
Nicolescu AC, Ji Y, Comeau JL, Hill BC, Takahashi T, Brien JF, Racz WJ, Massey TE.
Direct mitochondrial dysfunction precedes reactive oxygen species production in amiodarone-induced toxicity in human peripheral lung epithelial HPL1A cells
Toxicol Appl Pharmacol
2008
227(3):370-9
PubMed ID: 18191165
DOI: 10.1016/j.taap.2007.12.009
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16246
Nicolescu AC, Comeau JL, Hill BC, Bedard LL, Takahashi T, Brien JF, Racz WJ, Massey TE
Aryl radical involvement in amiodarone-induced pulmonary toxicity: investigation of protection by spin-trapping nitrones
Toxicol Appl Pharmacol
2007
220(1):60-71
PubMed ID: 17316728
DOI: 10.1016/j.taap.2006.12.031
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16245
Sato H, Yazawa T, Suzuki T, Shimoyamada H, Okudela K, Ikeda M, Hamada K, Yamada-Okabe H, Yao M, Kubota Y, Takahashi T, Kamma H, Kitamura H.
Growth regulation via insulin-like growth factor binding protein-4 and -2 in association with mutant K-ras in lung epithelia
Am J Pathol
2006
169(5):1550-66
PubMed ID: 17071580
DOI: 10.2353/ajpath.2006.051068
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16244
Martinez JM, Afshari CA, Bushel PR, Masuda A, Takahashi T, Walker NJ.
Differential toxicogenomic responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin in malignant and nonmalignant human airway epithelial cells
Toxicol Sci
2002
69(2):409-23
PubMed ID: 12377990
DOI: 10.1093/toxsci/69.2.409
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16243
Masuda A, Kondo M, Saito T, Yatabe Y, Kobayashi T, Okamoto M, Suyama M, Takahashi T, Takahashi T.
Establishment of human peripheral lung epithelial cell lines (HPL1) retaining differentiated characteristics and responsiveness to epidermal growth factor, hepatocyte growth factor, and transforming growth factor beta1
Cancer Res
1997
57(21):4898-904
PubMed ID: 9354455
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