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Cell No. : Cell Name
RCB5514 : Neuro2a-tNhtt-EGFP-150Q  update : 2024/02/07
CommentCurrently not ready.
Comment from the depositorStably transfected neuro2a cells, which inducibly express N-terminal Htt (exon 1) containing 16Q, 60Q or 150Q fusing EGFP (Neuro2a-tNhtt-EGFP-16Q, Neuro2a-tNhtt-EGFP-60Q, Neuro2a-tNhtt-EGFP-150Q) under control of ponasterone A. Neuro2a-RXR is the parental cell line of Neuro2a-tNhtt-EGFP cell lines before transfection of their expression vectors.
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Reference
16394  Yamanaka T, Wong HK, Tosaki A, Bauer PO, Wada K, Kurosawa M, Shimogori T, Hattori N, Nukina N.  Large-scale RNA interference screening in mammalian cells identifies novel regulators of mutant huntingtin aggregation  PLoS One  2014  9(4):e93891  PubMed ID: 24705917   DOI: 10.1371/journal.pone.0093891
16395  Bauer PO, Hudec R, Goswami A, Kurosawa M, Matsumoto G, Mikoshiba K, Nukina N.  ROCK-phosphorylated vimentin modifies mutant huntingtin aggregation via sequestration of IRBIT  Mol Neurodegener  2012  7:43  PubMed ID: 22929228   DOI: 10.1186/1750-1326-7-43
16396  Bauer PO, Hudec R, Ozaki S, Okuno M, Ebisui E, Mikoshiba K, Nukina N.  Genetic ablation and chemical inhibition of IP3R1 reduce mutant huntingtin aggregation  Biochem Biophys Res Commun  2011  416(1-2):13-7.  PubMed ID: 22056561   DOI: 10.1016/j.bbrc.2011.10.096
16397  Bauer PO, Goswami A, Wong HK, Okuno M, Kurosawa M, Yamada M, Miyazaki H, Matsumoto G, Kino Y, Nagai Y, Nukina N.  Harnessing chaperone-mediated autophagy for the selective degradation of mutant huntingtin protein  Nat Biotechnol  2010  28(3):256-63  PubMed ID: 20190739   DOI: 10.1038/nbt.1608
16398  Wong HK, Bauer PO, Kurosawa M, Goswami A, Washizu C, Machida Y, Tosaki A, Yamada M, Knöpfel T, Nakamura T, Nukina N.  Blocking acid-sensing ion channel 1 alleviates Huntington's disease pathology via an ubiquitin-proteasome system-dependent mechanism  Hum Mol Genet  2008  17(20):3223-35  PubMed ID: 18658163   DOI: 10.1093/hmg/ddn218
16399  Mitsui K, Doi H, Nukina N.  Proteomics of polyglutamine aggregates  Methods Enzymol  2006  412:63-76  PubMed ID: 17046652   DOI: 10.1016/S0076-6879(06)12005-4
16400  Nagaoka U, Kim K, Jana NR, Doi H, Maruyama M, Mitsui K, Oyama F, Nukina N.  Increased expression of p62 in expanded polyglutamine-expressing cells and its association with polyglutamine inclusions  J Neurochem  2004  91(1):57-68  PubMed ID: 15379887   DOI: 10.1111/j.1471-4159.2004.02692.x
16401  Doi H, Mitsui K, Kurosawa M, Machida Y, Kuroiwa Y, Nukina N.  Identification of ubiquitin-interacting proteins in purified polyglutamine aggregates  FEBS Lett  2004  571(1-3):171-6  PubMed ID: 15280037   DOI: 10.1016/j.febslet.2004.06.077
16402  Tanaka M, Machida Y, Niu S, Ikeda T, Jana NR, Doi H, Kurosawa M, Nekooki M, Nukina N.  Trehalose alleviates polyglutamine-mediated pathology in a mouse model of Huntington disease  Nat Med  2004  10(2):148-54.  PubMed ID: 14730359   DOI: 10.1038/nm985
16403  Zemskov EA, Nukina N.  Impaired degradation of PKCalpha by proteasome in a cellular model of Huntington's disease  Neuroreport  2003  14(11):1435-8  PubMed ID: 12960759   DOI: 10.1097/00001756-200308060-00006
16404  Mitsui K, Nakayama H, Akagi T, Nekooki M, Ohtawa K, Takio K, Hashikawa T, Nukina N.  Purification of polyglutamine aggregates and identification of elongation factor-1alpha and heat shock protein 84 as aggregate-interacting proteins  J Neurosci  2002  22(21):9267-77  PubMed ID: 12417652   DOI: 10.1523/JNEUROSCI.22-21-09267.2002
16405  Jana NR, Zemskov EA, Wang Gh, Nukina N.  Altered proteasomal function due to the expression of polyglutamine-expanded truncated N-terminal huntingtin induces apoptosis by caspase activation through mitochondrial cytochrome c release  Hum Mol Genet  2001  10(10):1049-59.  PubMed ID: 11331615   DOI: 10.1093/hmg/10.10.1049
16406  Jana NR, Tanaka M, Wang Gh, Nukina N.  Polyglutamine length-dependent interaction of Hsp40 and Hsp70 family chaperones with truncated N-terminal huntingtin: their role in suppression of aggregation and cellular toxicity  Hum Mol Genet  2000  9(13):2009-18  PubMed ID: 10942430   DOI: 10.1093/hmg/9.13.2009
16407  Wang GH, Mitsui K, Kotliarova S, Yamashita A, Nagao Y, Tokuhiro S, Iwatsubo T, Kanazawa I, Nukina N.  Caspase activation during apoptotic cell death induced by expanded polyglutamine in N2a cells  Neuroreport  1999  10(12):2435-8  PubMed ID: 10574348   DOI: 10.1097/00001756-199908200-00001

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