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Cell No. : Cell Name
RCB4943 : NU-RMESO-2  update : 2024/01/17
Comment
Comment from the depositorRat malignant mesothelioma induced by crocidolite peritoneal injection. Histology (tissue): epithelioid subtype, tubulopapillary variant. Histology (cell culture on the dish): solid-like. Immunohistochemistry: Podoplanin (-), Mesothelin (+), AE1/AE3 (+).
Terms and conditions1) A prior written permission of the approver is necessary for any kind of use including academic use and for-profit use. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Cancer Sci 2018 109(2):330-339) designated by the DEPOSITOR is requested. 3) In publishing the research results obtained by use of the cell, an acknowledgement is requested.
Remarks
approver's address
×
English
Address
Nagoya University Graduate School of Medicine
Department of Pathology and Biological Responses
65 Tsuruma-Cho, Showa-Ku, Nagoya, Aichi 466-8550
Dr.Ohara, Yuuki
Fax. +81-52-744-2091
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor OHARA, Yuuki
Originator OHARA, Yuuki & TOYOKUNI, Shinya
Year of deposit 2018
Animal _rat < Mammals
Strain name F1 hybrid rats between the Fischer-344 and Brown-Norway
Gender Male
Age at sampling 99 weeks
Tissue ascites
Primary focus peritoneal mesothelium
Disease name malignant mesothelioma
Metastatic ability No
Genetics Rat malignant mesothelioma induced by crocidolite peritoneal injection.
Classification cancer
Year of origin 2016
Lifespan infinite
Morphology epithelial-like
Immunological Podoplanin:Negative, Mesothelin:Positive, AE1/AE3:Positive
Cellosaurus(Expasy) CVCL_A6JJ
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium RPMI-1640 (Wako, 18902025), 1-10% fetal bovine serum (Funakoshi) RPMI1640 + 10% FBS
Antibiotics 1% antibiotic-antimycotic solution (Nacalai Tesque, 09366-44) Free
Passage method trypsin or scraper 0.25% Trypsin
Culture information Passage ratio 1 : 5-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Cell banker (ZENOGEN PHARMA, CB011) CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
11039  Yuuki Ohara, Shan-Hwu Chew, Takahiro Shibata, Yasumasa Okazaki, Kyoko Yamashita, Shinya Toyokuni  Phlebotomy as a preventive measure for crocidolite-induced mesothelioma in male rats  Cancer Sci  2018  109(2):330-339  PubMed ID: 29193587   DOI: 10.1111/cas.13460

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