Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB4936 : CT27  update : 2025/04/21
CommentTS culture protocol, TS culture protocol_V2
Comment from the depositorHuman trophoblast stem cells were derived from blastocysts or placental cytotrophoblast cells. Human trophoblast stem cells have the potential to differentiate into extravillous cytotrophoblast and syncytiotrophoblast cells.
Terms and conditions1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Stem Cell. 2018 ;22(1):50-63) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ARIMA, Takahiro
Originator ARIMA, Takahiro
Year of deposit 2018
Another name TS^(CT)#1
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue placenta (Cytotrophoblast cells)
Classification normal
Recombinant non-recombinant
Lifespan finite
Crisis PDL 150 PDL
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A7AZ
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium Please check [Comment]. Please check [Comment].
Antibiotics Free
Passage method Please check [Comment]. Please check [Comment].
Culture information Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER 1 CELLBANKER 1
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Mycoplasma (-)
Animal PCR OK
Virus (HIV) (-)
Virus (HTLV-1) (-)
Virus (HBV) (-)
Virus (HCV) (-)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 14


To topTop
Reference
4606  Takahashi S, Okae H, Kobayashi N, Kitamura A, Kumada K, Yaegashi N, Arima T.  Loss of p57KIP2 expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells.  Proc Natl Acad Sci U S A  2019  116(52):26606-26613  PubMed ID: 31792181   DOI: 10.1073/pnas.1916019116
4566  Okae H, Toh H, Sato T, Hiura H, Takahashi S, Shirane K, Kabayama Y, Suyama M, Sasaki H, Arima T.  Derivation of Human Trophoblast Stem Cells.  Cell Stem Cell.  2018  22:50-63  PubMed ID: 29249463   DOI: 10.1016/j.stem.2017.11.004

To topTop
User's Publication
22583  Sun C, Chamley LW, James JL.  Organoid generation from trophoblast stem cells highlights distinct roles for cytotrophoblasts and stem cells in organoid formation and expansion.  Placenta  2025    PubMed ID: 39658451   DOI: 10.1016/j.placenta.2024.12.003
19926  Asano T, Sasse P, Nakata T.  Development of a Cre-recombination-based color-switching reporter system for cell fusion detection  Biochem Biophys Res Commun  2024  690:149231  PubMed ID: 38000293   DOI: 10.1016/j.bbrc.2023.149231
21593  Shannon MJ, McNeill GL, Koksal B, Baltayeva J, Wächter J, Castellana B, Peñaherrera MS, Robinson WP, Leung PCK, Beristain AG.  Single-cell assessment of primary and stem cell-derived human trophoblast organoids as placenta-modeling platforms.  Dev Cell  2024    PubMed ID: 38359834   DOI: 10.1016/j.devcel.2024.01.023
21917  Hernández-Díaz N, Tzouganatou S, Mulik PR, Balestrini PA, Fogarty NME.  Transcriptional insights from the human embryo identify laminin-511 as a suitable matrix for human trophoblast stem cell culture  Placenta  2024  S0143-4004(24)00602-7  PubMed ID: 39095275   DOI: 10.1016/j.placenta.2024.07.308
22376  Logsdon DM, Ming H, Ezashi T, West RC, Schoolcraft WB, Roberts RM, Jiang Z, Yuan Y.  Transcriptome comparisons of trophoblasts from regenerative cell models with peri-implantation human embryos†  Biol Reprod  2024  111(5):1000-1016  PubMed ID: 39109839   DOI: 10.1093/biolre/ioae120
22831  S Tzouganatou, P Balestrini, N Fogarty  P-800 Best matrix match: Laminin-511 improves human trophoblast stem cell identity in vitro  Human Reproduction  2024  39    DOI: 10.1093/humrep/deae108.1111
19851  Baird L, Cannon P, Kandel M, Nguyen TV, Nguyen A, Wong G, Murphy C, Brownfoot FC, Kadife E, Hannan NJ, Tong S, Bartho LA, Kaitu'u-Lino TJ.  Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia  Reprod Biol Endocrinol  2023  21(1):65  PubMed ID: 37464405   DOI: 10.1186/s12958-023-01116-3
19852  Nguyen A, Cannon P, Kandel M, Nguyen TV, Baird L, Wong G, Hannan NJ, Tong S, Bartho L, Kaitu'u-Lino TJ.  Cell surface associated protein mucin 15 (MUC15) is elevated in preeclampsia  Placenta  2023  140:39-46  PubMed ID: 37531748   DOI: 10.1016/j.placenta.2023.07.013
19853  Kouthouridis S, Sotra A, Khan Z, Alvarado J, Raha S, Zhang B.  Modeling the Progression of Placental Transport from Early- to Late-Stage Pregnancy by Tuning Trophoblast Differentiation and Vascularization  Adv Healthc Mater  2023  12(32):e2301428  PubMed ID: 37830445   DOI: 10.1002/adhm.202301428
19854  Bartho LA, Kandel M, Walker SP, Cluver CA, Hastie R, Bergman L, Pritchard N, Cannon P, Nguyen TV, Wong GP, MacDonald TM, Keenan E, Hannan NJ, Tong S, Kaitu'u-Lino TJ.  Circulating Chemerin Is Elevated in Women With Preeclampsia  Endocrinology  2023  164(5):bqad041  PubMed ID: 36882076   DOI: 10.1210/endocr/bqad041
19940  Ogushi S, Nakanishi T, Kimura T.  Cadmium inhibits differentiation of human trophoblast stem cells into extravillous trophoblasts and disrupts epigenetic changes within the promoter region of the HLA-G gene  Toxicol Sci  2023  191(1):25-33  PubMed ID: 36370079   DOI: 10.1093/toxsci/kfac118
21346  Peng YH, Hsiao SK, Gupta K, Ruland A, Auernhammer GK, Maitz MF, Boye S, Lattner J, Gerri C, Honigmann A, Werner C, Krieg E.  Dynamic matrices with DNA-encoded viscoelasticity for cell and organoid culture.  Nat Nanotechnol  2023    PubMed ID: 37550574   DOI: 10.1038/s41565-023-01483-3
17331  Mizutani T, Orisaka M, Miyazaki Y, Morichika R, Uesaka M, Miyamoto K, Yoshida Y.  Inhibition of YAP/TAZ-TEAD activity induces cytotrophoblast differentiation into syncytiotrophoblast in human trophoblast  Mol Hum Reprod  2022  28(10):gaac032  PubMed ID: 35993908   DOI: 10.1093/molehr/gaac032
12302  Zhuosi Li, Osamu Kurosawa, Hiroo Iwata  A comparative study of key physiological stem cell parameters between three human trophoblast cell lines  Biochem Biophys Res Commun  2020  525(4):1038-1045  PubMed ID: 32178868   DOI: 10.1016/j.bbrc.2020.03.031


Back Back Return Top Page