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Cell No. : Cell Name
RCB4692 : HCT116-vim  update : 2024/01/17
Comment
Comment from the depositorTransgenic human cell line derived from colon cancer. This cell line is suitable to observe epithelial- mesenchymal transformation.
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Department of Gastroenterological Surgery,
Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
2-5-1 Shikata-cho, Kitaku, Okayama 700-8558 Japan
Dr.Fujiwara Toshiyoshi
Fax. +81-86-235-7492
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor FUJIWARA, Toshiyoshi
Originator IEDA, Takeshi
Original cell HCT116
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Recombinant recombinant
Exogene human vimentin promoter
6.3K base
Vector pTurboFP635
Selection 600 μg/ml Geneticin
Recombinant virus No
Lifespan infinite
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium McCoy's 5A (gibco) +10% Foetal Bovine Serum (SIGMA) McCoy's 5A +10% FBS + 600 μg/ml Geneticin
Antibiotics 1% Penicillin-Streptomycin (SIGMA)
Passage method 0.25% Trypsin
Designated culture dish T25
Culture information Passage cell No 25-75 x 10,000 cells/plate 2.5-7.5x10 5 cells/T25 flask
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER 1 CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
6832  Ieda T, Tazawa H, Okabayashi H, Yano S, Shigeyasu K, Kuroda S, Ohara T, Noma K, Kishimoto H, Nishizaki M, Kagawa S, Shirakawa Y, Saitou T, Imamura T, Fujiwara T.  Visualization of epithelial-mesenchymal transition in an inflammatory microenvironment-colorectal cancer network.  Sci Rep  2019  9(1):16378  PubMed ID: 31705021   DOI: 10.1038/s41598-019-52816-z

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