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Cell No. : Cell Name
RCB4684 : celf3  update : 2024/01/17
Comment
Comment from the depositorAnti-Celf3 antibody production hybridoma cell
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genes Cells. 2014;19:704-721) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nakagawa, Shinichi
Originator Ishizuka, Akira
Year of deposit 2016
Animal _mouse x mouse < Hybrid
Classification hybrid cells
Recombinant non-recombinant
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_A7DJ
hybridoma Fusion_cell_1 name P3U1
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_1 gender Unknown
Fusion_cell_2 name lymphocyte
Fusion_cell_2 strain mouse, ICR
Fusion_cell_2 gender Female
Fusion method 50% polyethylene glycol, HAT, ELIZA, Western blotting
Antibody name Anti-celf3
Subclass IgG
Antigen_name celf3
Antigen_positive nervous system
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium GIT + 1ng/ml IL6 GIT + 1ng/ml mouse IL-6
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER CELLBANKER2
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
4022  Ishizuka A, Hasegawa Y, Ishida K, Yanaka K, Nakagawa S.  Formation of nuclear bodies by the lncRNA Gomafu-associating proteins Celf3 and SF1.  Genes Cells  2014  19:704-721  PubMed ID: 25145264   DOI: 10.1111/gtc.12169

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