Cell No. : Cell Name
RCB4662 : HCT116 CMV-OsTIR1
update : 2023/10/16
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Comment | cf.: Kanemaki's Auxin Inducible Degron (AID) System |
Comment from the depositor | This is an HCT116 cell line in which CMV-OsTIR1 (pMK232, PURO) was introduced at the both alleles of the AAVS1 locus by using CRISPR/Cas.This parental cell line consitutively express OsTIR1 and can be used for generation of AID mutants. |
Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Rep 2016;15:210-218) designated by the DEPOSITOR is required. |
Remarks | CRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology. |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0065.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Kanemaki, Masato
|
Originator |
Kanemaki, Masato
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Year of deposit |
2016
|
Animal |
_human
< Mammals
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Genus |
Homo
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Species |
sapiens
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Gender |
Male
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Tissue |
colon
|
Classification |
cancer
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Recombinant |
recombinant
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Exogene |
CMV-OsTIR1, pMK232, PURO
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|
Lifespan |
infinite
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Morphology |
epithelial-like
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Cellosaurus(Expasy) |
CVCL_RJ11
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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Culture medium |
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McCoy's 5A (with Glutamine) + 2mM L-Glutamine + 10% FBS
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Antibiotics |
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free
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Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 10 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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Animal PCR |
|
OK
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STR(human) |
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OK
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Images |
deposit info | lot info |
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Reference information |
Reference |
3
|
User's Publication |
0
|
Reference |
4031
Tungadi EA, Ito A, Kiyomitsu T, Goshima G.
Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2
J Cell Sci
2017
130(21):3676-3684
PubMed ID: 28883092
DOI: 10.1242/jcs.203703
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4030
Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT.
Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors
Cell Rep
2016
15:210-218
PubMed ID: 27052166
DOI: 10.1016/j.celrep.2016.03.001
|
5491
Brattain MG, Fine WD, Khaled FM, Thompson J, Brattain DE.
Heterogeneity of malignant cells from a human colonic carcinoma.
Cancer Res
1981
41(5):1751-6
PubMed ID: 7214343
|