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Cell No. : Cell Name
RCB4662 : HCT116 CMV-OsTIR1  update : 2023/10/16
Commentcf.: Kanemaki's Auxin Inducible Degron (AID) System
Comment from the depositorThis is an HCT116 cell line in which CMV-OsTIR1 (pMK232, PURO) was introduced at the both alleles of the AAVS1 locus by using CRISPR/Cas.This parental cell line consitutively express OsTIR1 and can be used for generation of AID mutants.
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Rep 2016;15:210-218) designated by the DEPOSITOR is required.
RemarksCRISPR/Cas9 genome edited bioresources Announcement of bioresources developed by the CRISPR/Cas9 technology.
Order Form Order Form(C-0005.pdf)   MTA(C-0065.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kanemaki, Masato
Originator Kanemaki, Masato
Year of deposit 2016
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue colon
Classification cancer
Recombinant recombinant
Exogene CMV-OsTIR1, pMK232, PURO
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_RJ11
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium McCoy's 5A (with Glutamine) + 2mM L-Glutamine + 10% FBS
Antibiotics free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
4031  Tungadi EA, Ito A, Kiyomitsu T, Goshima G.  Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2  J Cell Sci  2017  130(21):3676-3684  PubMed ID: 28883092   DOI: 10.1242/jcs.203703
4030  Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT.  Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors  Cell Rep  2016  15:210-218  PubMed ID: 27052166   DOI: 10.1016/j.celrep.2016.03.001
5491  Brattain MG, Fine WD, Khaled FM, Thompson J, Brattain DE.  Heterogeneity of malignant cells from a human colonic carcinoma.  Cancer Res  1981  41(5):1751-6  PubMed ID: 7214343  

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