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RCB4627 : KUP5  update : 2018/07/06
CommentAn immortalized mouse Kupffer cell line established from C57BL/6 strain. The Kupffer cells were proliferated in the mixed primary culture of C57BL/6 adult mouse liver cells, then infected with a replication-deficient retroviral vector containing the human c-myc oncogene and neomycin resistance gene, isolated and selected with G418 (cloned by a limited dilution).
Comment from the depositor
Terms and conditions1) A permission of the approver is required. 2) If any intellectual Property is generated during the use of the delivered Material, the Recipient shall consult with the Administrator of the Material at the NARO. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Results in Immunology 2014;4:68-74) designated by the DEPOSITOR is required.
approver's address
The National Agriculture and Food Research Organization
Institute of Agrobiological Sciences, Division of Animal Science
1-2 Owashi, Tsukuba, Ibaraki 305-8634 Japan
Dr.Kitani, Hiroshi
Fax. +81-29-838-6043
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Basic information Depositor Kitani, Hiroshi
Originator Kitani, Hiroshi
Year of deposit 2015
Animal mouse < Mammals
Strain name C57BL/6
Genus Mus
Species musculus
Gender Male
Age at sampling 7 weeks
Tissue liver
Classification transformed
Recombinant recombinant
Exogene replication-deficient retroviral vector , human c-myc, Neomycin resistance gene, HSV-TK
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS + 10μg/ml bovine Insulin + 250μM Monothioglycerol
Antibiotics Free
Passage method TrypLE Express
Designated culture dish FALCON 1008
Culture information Passage cell No 2-5 x 10^(5) cells/100 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP (mouse) OK
deposit info
lot info
Reference information Reference 1
User's Publication 3

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4025  Establishment of c-myc-immortalized Kupffer cell line from a C57BL/6 mouse strain.  Kitani H, Sakuma C, Takenouchi T, Sato M, Yoshioka M, Yamanaka N.  Results Immunol  2014  4:68-74  PubMed ID: 25379377

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User's Publication
10619  Red blood cell-like particles with the ability to avoid lung and spleen accumulation for the treatment of liver fibrosis.  Hayashi K, Yamada S, Hayashi H, Sakamoto W, Yogo T.  Biomaterials  2018  156:45-55  PubMed ID: 29190497
10835  Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice.  Kim KH, Kim SH, Han DH, Jo YS, Lee YH, Lee MS.  Sci Rep  2018  8:6789  PubMed ID: 29717162
11054  Microbiome inhibition of IRAK-4 by trimethylamine mediates metabolic and immune benefits in high-fat-diet-induced insulin resistance  J Chilloux, F Brial, et al  bioRxiv  2018   

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