An immortalized mouse Kupffer cell line established from C57BL/6 strain. The Kupffer cells were proliferated in the mixed primary culture of C57BL/6 adult mouse liver cells, then infected with a replication-deficient retroviral vector containing the human c-myc oncogene and neomycin resistance gene, isolated and selected with G418 (cloned by a limited dilution).
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The National Agriculture and Food Research Organization Institute of Agrobiological Sciences, Division of Animal Science 1-2 Owashi, Tsukuba, Ibaraki 305-8634 Japan
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replication-deficient retroviral vector , human c-myc, Neomycin resistance gene, HSV-TK
Establishment of c-myc-immortalized Kupffer cell line from a C57BL/6 mouse strain.
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