Cell No. : Cell Name RCB4623 : Ba/ER  update : 2024/01/17
Comment			Ba/ER was created by stable transfection of murine pro-B Ba/F3 cells with a murine erythropoietin receptor gene. Ba/ER proliferates dependent on erythropoietin or interleukin-3.
Comment from the depositor
Terms and conditions			In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Immunol Methods 2000;241:159-170) designated by the DEPOSITOR is required.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Kawahara, Masahiro
		Originator	Kawahara, Masahiro
		Year of deposit	2015
		Animal	_mouse < Mammals
		Tissue	blood
		Classification	transformed
		Recombinant	recombinant
		Exogene	pME-ER (murine erythropoietin receptor gene), neo
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_6C87
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture medium		RPMI1640 + 10% FBS + (1ng/ml mouse IL-3 or 3U/ml human EPO)
		Culture method
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		SSLP(mouse)		OK
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	0

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Reference

3665  Ueda H, Kawahara M, Aburatani T, Tsumoto K, Todokoro K, Suzuki E, Nishimura H, Schueler PA, Winter G, Mahoney WC, Kumagai I, Nagamun  Cell-growth control by monomeric antigen: the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor.  J Immunol Methods  2000  241(1-2):159-70  PubMed ID: 10915858   DOI: 10.1016/s0022-1759(00)00202-7

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