Cell No. : Cell Name
RCB4563 : TSt-4/N-DLL4
update : 2024/01/17
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| Comment | Original murine mesenchymal cell line was derived from fetal thymus tissue. A line Expressing DLL4 gene. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Science 2010;329:93-96. ) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kawamoto, Hiroshi
|
| Originator |
Kawamoto, Hiroshi
|
| Year of deposit |
2014
|
| Animal |
_mouse
< Mammals
|
| Strain name |
BALB/c
|
| Age at sampling |
fetus
|
| Tissue |
thymus
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
MSCV-IRES-NGFR(human), murine DLL4 (delta-like 4)
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_VP74
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
RPMI1640 + 5% FBS
|
RPMI1640 + 5% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
1 : 4 split
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
(-)
|
(-)
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
0
|
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