Cell No. : Cell Name
RCB4561 : MOLT-17
update : 2025/01/16
|
| Comment | Human cell line derived from T cell leukemia (T-ALL). Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to DSMZ no.:
ACC 36 prior to order. |
| Comment from the depositor | |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ACC 36 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
DSMZ
|
| Year of deposit |
2014
|
| Original cell |
ACC 36
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
5 years
|
| Tissue |
Peripheral blood lymphocytes
|
| Classification |
cancer
|
| History |
Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan → DSMZ（ACC 36）
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_2125
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS (heat inactivated)
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 8-16 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
5516
Drexler HG1, Minowada J.
Morphological, immunophenotypical and isoenzymatic profiles of human leukemia cells and derived T-cell lines.
Hematol Oncol
1989
7(2):115-25.
PubMed ID: 2784121
DOI: 10.1002/hon.2900070203
|
|
3544
Tighe L, Forster A, Clark DM, Boylston AW, Lavenir I, Rabbitts TH.
Unusual forms of T cell gamma mRNA in a human T cell leukemia cell line: implications for gamma gene expression.
Eur J Immunol
1987
17(12):1729-36
PubMed ID: 2961573
DOI: 10.1002/eji.1830171208
|
|
2232
DeCoursey TE, Chandy KG, Gupta S, Cahalan MD.
Voltage-dependent ion channels in T-lymphocytes
J Neuroimmunol
1985
10(1):71-95
PubMed ID: 2414315
DOI: 10.1016/0165-5728(85)90035-9
|
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