細胞番号 : 細胞名
RCB4548 : PT-r
update : 2023/10/16
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| 細胞特性(Comment:英) | Rat cell line derived from parathyroid gland. |
| 細胞特性(日) | ラット副甲状腺由来細胞株。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | 1) The availability of the BIOLOGICAL RESOUSE is limited to a RECIPIENT of a non-for-profit organization for a not-for-profit research. 2) Distribution of the BIOLOGICAL RESOUCE to a third party without permission is prohibited. 3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Proc Natl Acad Sci USA 1987;84:3269-3273, J Biol Chem 1989;264:15714-15720, J Biol Chem 1990;265:13661-13668, J Biol Chem 1992;267:24554-24562, Life Sci 2008;82:677-683, Bone 2010;47:534-541) . 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| 使用条件(日) | 1) 学術機関の学術研究に限る。 2) 第三者への無断譲渡を禁止する。 3) 利用者が本リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 4) 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Proc Natl Acad Sci USA 1987;84:3269-3273、J Biol Chem 1989;264:15714-15720、J Biol Chem 1990;265:13661-13668、J Biol Chem 1992;267:24554-24562、Life Sci 2008;82:677-683、Bone 2010;47:534-541) を引用すること。 5) 利用者は、成果の公表にあたって謝辞の表明を必要とする。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Sakaguchi, Kazushige
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| 樹立者 |
Sakaguchi, Kazushige
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| 寄託日 |
2013
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| 動物種 |
_rat
< Mammals
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| 属名 |
Rattus
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| 種名 |
norvegicus
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| 性別 |
Male
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| 齢 |
3 months
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| 採取組織 |
parathyroid gland
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| 細胞分類 |
normal
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細胞寿命 |
infinite
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| 細胞形態 |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_HF63
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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Keratinocyte-SFM + 10mM HEPES
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA), added Keratinocyte-SFM+10%FBS, washed twice with medium
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播種細胞数 |
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2 x 10 5 cells/100 mm dish
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| 継代・培地交換頻度 |
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Subculture : 2 times/week (not confluent)
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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CELLBANKER 2
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| 動物種PCR |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
13件
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| Reference(日) |
0件
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| 利用者成果(英) |
0件
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| 利用者成果(日) |
0件
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| Reference(英) |
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4569
Kawahara M, Iwasaki Y, Sakaguchi K, Taguchi T, Nishiyama M, Nigawara T, Kambayashi M, Sawada T, Jing X, Miyajima M, Terada Y, Hashimoto K, Suda T.
Involvement of GCMB in the transcriptional regulation of the human parathyroid hormone gene in a parathyroid-derived cell line PT-r: effects of calcium and 1,25(OH)2D3.
Bone
2010
47:534-41
PubMed ID: 20558332
DOI: 10.1016/j.bone.2010.05.031
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3654
Kawahara M, Iwasaki Y, Sakaguchi K, Taguchi T, Nishiyama M, Nigawara T, Tsugita M, Kambayashi M, Suda T, Hashimoto K.
Predominant role of 25OHD in the negative regulation of PTH expression: clinical relevance for hypovitaminosis D.
Life Sci
2008
82(13-14):677-83
PubMed ID: 18304585
DOI: 10.1016/j.lfs.2007.12.027
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3659
Fujii Y, Tomić M, Stojilković SS, Iida T, Brandi ML, Ogino Y, Sakaguchi K.
Effects of endothelin-1 on Ca2+ signaling and secretion in parathyroid cells.
J Bone Miner Res
1995
10(5):716-25
PubMed ID: 7639107
DOI: 10.1002/jbmr.5650100508
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3658
Takagi Y, Shrivastav S, Miki T, Sakaguchi K.
Molecular cloning and expression of the acidic fibroblast growth factor receptors in a rat parathyroid cell line (PT-r). Parathyroid cell-specific calcium-dependent change of ligand accessibility and covalent attachment of heparan sulfate glycosaminoglycan to the receptors.
J Biol Chem
1994
269(38):23743-9
PubMed ID: 8089146
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873
Bianchi S, Fabiani S, Muratori M, Arnold A, Sakaguchi K, Miki T, Brandi ML.
Calcium modulates the cyclin D1 expression in a rat parathyroid cell line
Biochem Biophys Res Commun
1994
204(2):691-700
PubMed ID: 7980531
DOI: 10.1006/bbrc.1994.2515
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3653
Sakaguchi K.
Acidic fibroblast growth factor autocrine system as a mediator of calcium-regulated parathyroid cell growth.
J Biol Chem
1992
267(34):24554-62
PubMed ID: 1280262
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3657
Sakaguchi K, Yanagishita M, Takeuchi Y, Aurbach GD.
Identification of heparan sulfate proteoglycan as a high affinity receptor for acidic fibroblast growth factor (aFGF) in a parathyroid cell line.
J Biol Chem
1991
266(11):7270-8
PubMed ID: 1707883
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872
Fujii Y, Moreira JE, Orlando C, Maggi M, Aurbach GD, Brandi ML, Sakaguchi K.
Endothelin as an autocrine factor in the regulation of parathyroid cells
Proc Natl Acad Sci U S A
1991
88(10):4235-9
PubMed ID: 1852000
DOI: 10.1073/pnas.88.10.4235
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3652
Takeuchi Y, Sakaguchi K, Yanagishita M, Aurbach GD, Hascall VC.
Extracellular calcium regulates distribution and transport of heparan sulfate proteoglycans in a rat parathyroid cell line.
J Biol Chem
1990
265(23):13661-8
PubMed ID: 2143187
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3656
Sakaguchi K, Ikeda K, Curcio F, Aurbach GD, Brandi ML.
Subclones of a rat parathyroid cell line (PT-r): regulation of growth and production of parathyroid hormone-related peptide (PTHRP).
J Bone Miner Res
1990
5(8):863-9
PubMed ID: 1700579
DOI: 10.1002/jbmr.5650050810
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3651
Yanagishita M, Brandi ML, Sakaguchi K.
Characterization of proteoglycans synthesized by a rat parathyroid cell line.
J Biol Chem
1989
264(26):15714-20
PubMed ID: 2768284
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871
Ikeda K, Weir EC, Sakaguchi K, Burtis WJ, Zimering M, Mangin M, Dreyer BE, Brandi ML, Aurbach GD, Broadus AE.
Clonal rat parathyroid cell line expresses a parathyroid hormone-related peptide but not parathyroid hormone itself
Biochem Biophys Res Commun
1989
162(1):108-15
PubMed ID: 2751644
DOI: 10.1016/0006-291x(89)91969-4
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4568
Sakaguchi K, Santora A, Zimering M, Curcio F, Aurbach GD, Brandi ML.
Functional epithelial cell line cloned from rat parathyroid glands.
Proc Natl Acad Sci U S A
1987
84:3269-73
PubMed ID: 3472208
DOI: 10.1073/pnas.84.10.3269
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