Cell No. : Cell Name
RCB4485 : A2F10
update : 2024/01/17
|
| Comment | Hybridoma cell line producing anti-mouse Flk-2/Flt-3 (CD135) antibody. |
| Comment from the depositor | |
| Terms and conditions | 1) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purpose only. (Commercial use is prohibited by the DEPOSITOR.) 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Exp Hematol 1998;26:478-488) is necessary. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nishikawa, Shin-ichi
|
| Originator |
Ogawa, Minetaro
|
| Year of deposit |
2012
|
| Another name |
anti mouse Flk-2/Flk-3
|
| Animal |
_mouse x rat
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_KA98
|
|
hybridoma
|
Fusion_cell_1 name |
spleen cells
|
| Fusion_cell_1 strain |
rat, Wistar
|
| Fusion_cell_2 name |
X63-Ag8/653
|
| Fusion_cell_2 strain |
mouse
|
|
Fusion method |
PEG #4000, HAT selection
|
|
Antibody name |
A2F10
|
| Subclass |
rat IgG2a
|
| Immunogen |
Rat myeloma cell line Y3 which the murine flt-3 gene is introduced.
|
|
Antigen_name |
Flk2/Flt3
|
| Antigen_m.w. |
150kDa
|
| Antigen_character |
transmembrane tyrosin-kinase receptor
|
|
Antigen_positive |
early hematopoietic progenitor
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture medium |
|
RPMI1640 + 10% FBS + 50μM 2-Mercaptoethanol
|
| Culture method |
|
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
90% FBS + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Antibody subclass |
|
rat IgG2a
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
0
|
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