Cell No. : Cell Name RCB4476 : Ba/F3  update : 2024/09/20
Comment			Mouse pro-B cell line derived from C3H strain. IL-3 dependent. Information
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Tani, Kenzaburo
		Year of deposit	2012
		Animal	_mouse < Mammals
		Strain name	C3H
		Tissue	bone marrow
		Classification	other
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_0161
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS + 10ng/ml mouse IL-3
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 3 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		SSLP(mouse)		OK
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	7

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Reference

4829  Daley GQ1, Baltimore D.  Transformation of an interleukin 3-dependent hematopoietic cell line by the chronic myelogenous leukemia-specific P210bcr/abl protein.  Proc Natl Acad Sci U S A  1988  85(23):9312-6  PubMed ID: 3143116   DOI: 10.1073/pnas.85.23.9312

4828  Palacios R, Steinmetz M.  Il-3-dependent mouse clones that express B-220 surface antigen, contain Ig genes in germ-line configuration, and generate B lymphocytes in vivo.  Cell  1985  41(3):727-34  PubMed ID: 3924409   DOI: 10.1016/s0092-8674(85)80053-2

4827  Palacios R, Henson G, Steinmetz M, McKearn JP.  Interleukin-3 supports growth of mouse pre-B-cell clones in vitro.  Nature  1984  309(5964):126-31  PubMed ID: 6201749   DOI: 10.1038/309126a0

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User's Publication

21874  Ochi N, Miyake N, Takeyama M, Yamane H, Fukazawa T, Nagasaki Y, Kawahara T, Ichiyama N, Kosaka Y, Mimura A, Nakanishi H, Hiraki A, Kiura K, Takigawa N.  The combined inhibition of SLC1A3 and glutaminase in osimertinib-resistant EGFR mutant cells.  Biochim Biophys Acta Gen Subj  2024  1868(10):130675  PubMed ID: 39059510   DOI: 10.1016/j.bbagen.2024.130675

22096  Watanabe D, Hiroshima M, Yasui M, Ueda M.  Single molecule tracking based drug screening.  Nat Commun  2024  15(1):8975  PubMed ID: 39420015   DOI: 10.1038/s41467-024-53432-w

21413  Miyazaki I, Odintsov I, Ishida K, Lui AJW, Kato M, Suzuki T, Zhang T, Wakayama K, Kurth RI, Cheng R, Fujita H, Delasos L, Vojnic M, Khodos I, Yamada Y, Ishizawa K, Mattar MS, Funabashi K, Chang Q, Ohkubo S, Yano W, Terada R, Giuliano C, Lu YC, Bonifacio A, Kunte S, Davare MA, Cheng EH, de Stanchina E, Lovati E, Iwasawa Y, Ladanyi M, Somwar R.  Vepafestinib is a pharmacologically advanced RET-selective inhibitor with high CNS penetration and inhibitory activity against RET solvent front mutations.  Nat Cancer  2023  4(9):1345-1361  PubMed ID: 37743366   DOI: 10.1038/s43018-023-00630-y

20690  Miettinen TP, Ly KS, Lam A, Manalis SR.  Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis.  Elife  2022  11  PubMed ID: 35535854   DOI: 10.7554/eLife.76664

15835  Nishiya N, Oku Y, Ishikawa C, Fukuda T, Dan S, Mashima T, Ushijima M, Furukawa Y, Sasaki Y, Otsu K, Sakyo T, Abe M, Yonezawa H, Ishibashi F, Matsuura M, Tomida A, Seimiya H, Yamori T, Iwao M, Uehara Y.  Lamellarin 14, a derivative of marine alkaloids, inhibits the T790M/C797S mutant epidermal growth factor receptor  Cancer Sci  2021  112(5):1963-1974  PubMed ID: 33544933   DOI: 10.1111/cas.14839

13269  Kang JH, Katsikis G, Li Z, Sapp KM, Stockslager MA, Lim D, Vander Heiden MG, Yaffe MB, Manalis SR, Miettinen TP.  Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division.  Nat Commun  2020    PubMed ID: 33020492   DOI: 10.1038/s41467-020-18769-y

17098  Miettinen TP, Kang JH, Yang LF, Manalis SR.  Mammalian cell growth dynamics in mitosis  Elife  2019  8:e44700.  PubMed ID: 31063131   DOI: 10.7554/eLife.44700