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RCB3687 : MKN-7  update : 2018/08/02
CommentHuman cell line derived from stomach cancer (gastric cancer; highly differentiated tubular adenocarcinoma). TKG0228 (Deposited from Tohoku Univ.).
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Hojo, H.
Year of deposit 2011
Original cell TKG0228
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 39 years
Tissue stomach
Case history Highly differentiated tubular adenocarcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0228)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Culture information Passage ratio 1 : 3-4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
10867    Neuropilin 2 could promote gastric adenocarcinoma lymphatic invasionwith VEGF-C stimulation   Int J Clin Exp Med  2016  9:2795-2803 



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