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Cell No. : Cell Name
RCB3686 : THP-1  update : 2024/10/17
CommentHuman cell line derived from monocytic leukemia. TKG0267 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Tsuchiya, S.
Year of deposit 2011
Original cell TKG0267
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 1 year
Tissue peripheral blood
Disease name acute monocytic leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University（TKG0267）
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0006
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 12


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Reference
4951  Tsuchiya S, Minegishi N, Minegishi M, Sato T, Konno T.  Adaptation of a human monocytic leukemia cell line (THP-1) in a protein-free chemically defined medium.  Tohoku J Exp Med  1986  150(4):455-65  PubMed ID: 3551191   DOI: 10.1620/tjem.150.455
4950  Tajima S, Hayashi R, Tsuchiya S, Miyake Y, Yamamoto A.  Cells of a human monocytic leukemia cell line (THP-1) synthesize and secrete apolipoprotein E and lipoprotein lipase.  Biochem Biophys Res Commun  1985  126(1):526-31  PubMed ID: 3855620   DOI: 10.1016/0006-291x(85)90637-0
4949  Tsuchiya S, Kobayashi Y, Goto Y, Okumura H, Nakae S, Konno T, Tada K.  Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester.  Cancer Res  1982  42(4):1530-6  PubMed ID: 6949641  
720  Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K.  Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).  Int J Cancer  1980  26(2):171-6  PubMed ID: 6970727   DOI: 10.1002/ijc.2910260208

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User's Publication
19710  Johnson EL, Ohkawa Y, Kanto N, Fujinawa R, Kuribara T, Miyoshi E, Taniguchi N.  The S1 spike protein of SARS-CoV-2 upregulates the ERK/MAPK signaling pathway in DC-SIGN-expressing THP-1 cells  Cell Stress Chaperones  2024  29(2):227-234  PubMed ID: 38453000   DOI: 10.1016/j.cstres.2024.03.002
21946  Nakamura H, Hikichi H, Seto S, Hijikata M, Keicho N.  Transcriptional regulators SP110 and SP140 modulate inflammatory response genes in Mycobacterium tuberculosis-infected human macrophages.  Microbiol Spectr  2024  e0010124  PubMed ID: 39162523   DOI: 10.1128/spectrum.00101-24
19615  Nakahara R, Aki S, Sugaya M, Hirose H, Kato M, Maeda K, Sakamoto DM, Kojima Y, Nishida M, Ando R, Muramatsu M, Pan M, Tsuchida R, Matsumura Y, Yanai H, Takano H, Yao R, Sando S, Shibuya M, Sakai J, Kodama T, Kidoya H, Shimamura T, Osawa T.  Hypoxia activates SREBP2 through Golgi disassembly in bone marrow-derived monocytes for enhanced tumor growth  EMBO J  2023  42(22):e114032  PubMed ID: 37781951   DOI: 10.15252/embj.2023114032
19877  Nakatomi T, Itaya-Takahashi M, Horikoshi Y, Shimizu N, Parida IS, Jutanom M, Eitsuka T, Tanaka Y, Zingg JM, Matsura T, Nakagawa K.  The difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albumin  Sci Rep  2023  13(1):7392  PubMed ID: 37149706   DOI: 10.1038/s41598-023-34584-z
21183  Xiao L, Mochizuki M, Wang D, Shimamura N, Sunada K, Nakahara T.  Types of cell culture inserts affect cell crosstalk between co-cultured macrophages and adipocytes.  Biochem Biophys Res Commun  2023  658:10-17  PubMed ID: 37011478   DOI: 10.1016/j.bbrc.2023.03.068
21221  Fujita H, Adachi C, Inoue T.  Cholesterol-load evokes robust calcium response in macrophages: An early event toward cholesterol-induced macrophage death.  Cell Calcium  2023  113:102754  PubMed ID: 37196488   DOI: 10.1016/j.ceca.2023.102754
21364  Yamanaka S, Furihata H, Yanagihara Y, Taya A, Nagasaka T, Usui M, Nagaoka K, Shoya Y, Nishino K, Yoshida S, Kosako H, Tanokura M, Miyakawa T, Imai Y, Shibata N, Sawasaki T.  Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation.  Nat Commun  2023  14(1):4683  PubMed ID: 37596276   DOI: 10.1038/s41467-023-40385-9
20628  Xiao L, Mochizuki M, Fan Y, Nakahara T, Liao F.  Enzyme-digested Colla Corii Asini (E'jiao) suppresses lipopolysaccharide-induced inflammatory changes in THP-1 macrophages and OP9 adipocytes.  Hum Cell  2022    PubMed ID: 35359251   DOI: 10.1007/s13577-022-00694-5
22271  Hikichi H, Seto S, Wakabayashi K, Hijikata M, Keicho N.  Transcription factor MAFB controls type I and II interferon response-mediated host immunity in Mycobacterium tuberculosis-infected macrophages  Front Microbiol  2022  13:962306  PubMed ID: 36406405   DOI: 10.3389/fmicb.2022.962306
22272  Fukano H, Nakanaga K, Goto M, Yoshida M, Ishii N, Hoshino Y.  Therapeutic efficacy of rifalazil (KRM-1648) in a M. ulcerans-induced Buruli ulcer mouse model  PLoS One  2022  17(10):e0274742  PubMed ID: 36201529   DOI: 10.1371/journal.pone.0274742
20361  Okamoto Y, Kitakaze K, Takenouchi Y, Yamamoto S, Ishimaru H, Tsuboi K.  Sphingosine 1-phosphate receptor type 2 positively regulates interleukin (IL)-4/IL-13-induced STAT6 phosphorylation.  Cell Signal  2021  88:110156  PubMed ID: 34592416   DOI: 10.1016/j.cellsig.2021.110156
13052  Kawai S, Suzuki M, Arimoto S, Korenaga T, Yasukawa T.  Determination of membrane capacitance and cytoplasm conductivity by simultaneous electrorotation.  Analyst  2020    PubMed ID: 32462157   DOI: 10.1039/d0an00100g


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