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Cell No. : Cell Name
RCB3680 : HeLa  update : 2023/10/16
CommentHuman cell line derived from cervical cancer. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cycline and MC210. TKG0331 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Gey, G. O.
Year of deposit 2011
Original cell TKG0331
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervical carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0331)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0030
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 6


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Reference
1446  J E DARNELL Jr, T K SAWYERc  Variation in plaque-forming ability among parental and clonal strains of HeLa cells  Virology  1959  223-9  PubMed ID: 13669339   DOI: 10.1016/0042-6822(59)90006-6
1444  Puck TT1, Marcus PI.  A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS.  Proc Natl Acad Sci U S A  1955  41(7):432-7  PubMed ID: 16589695   DOI: 10.1073/pnas.41.7.432
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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User's Publication
17800  Asano-Inami E, Yokoi A, Sugiyama M, Hyodo T, Hamaguchi T, Kajiyama H.  The association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formation  Commun Biol  2023  6(1):415  PubMed ID: 37059803   DOI: 10.1038/s42003-023-04754-w
11381  Win NN, Kyaw MM, Prema, Ngwe H, Ito T, Asakawa Y, Okamoto Y, Tanaka M, Abe I, Morita H.  Dinorcassane Diterpenoid from Boesenbergia rotunda Rhizomes Collected in Lower Myanmar.  Chem. Biodivers.  2019    PubMed ID: 30645035   DOI: 10.1002/cbdv.201800657
12436  Ozeki K, Sugiyama M, Akter KA, Nishiwaki K, Asano-Inami E, Senga T.  FAM98A is localized to stress granules and associates with multiple stress granule-localized proteins  Mol Cell Biochem  2019  451(1-2):107-115  PubMed ID: 29992460   DOI: 10.1007/s11010-018-3397-6
9988  Li S, Gao A, Dong S, Chen Y, Sun S, Lei Z, Zhang Z.  Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.  Int. J. Biol. Macromol.  2017  96:26-34  PubMed ID: 27939510   DOI: 10.1016/j.ijbiomac.2016.12.007
9792  Hyodo T, Ito S, Asano-Inami E, Chen D, Senga T.  A regulatory subunit of protein phosphatase 2A, PPP2R5E, regulates the abundance of microtubule crosslinking factor 1.  FEBS J.  2016  283:3662-3671  PubMed ID: 27521566   DOI: 10.1111/febs.13835
15027  Dan Zhu, Hongyi Sun, Shuhong Li, Xuansheng Hu, Xi Yuan, Chao Han, Zhenya Zhang  Influence of Drying Methods on Antioxidant Activities and Immunomodulatory of Aqueous Extract From Soybean Curd Residue Fermentated by Grifola frondosa  International Journal of Biology  2014  7:82-92    DOI: 10.5539/ijb.v7n1p82



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