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RCB3680 : HeLa  update : 2018/08/02
CommentHuman cell line derived from cervical cancer. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cycline and MC210. TKG0331 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Gey, G. O.
Year of deposit 2011
Original cell TKG0331
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervical carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0331)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 4


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Reference
1444  Puck TT1, Marcus PI.  A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS.  Proc Natl Acad Sci U S A  1955  41(7):432-7  PubMed ID: 16589695
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265 

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User's Publication
11381  Win NN, Kyaw MM, Prema, Ngwe H, Ito T, Asakawa Y, Okamoto Y, Tanaka M, Abe I, Morita H.  Dinorcassane Diterpenoid from Boesenbergia rotunda Rhizomes Collected in Lower Myanmar.  Chem. Biodivers.  2019    PubMed ID: 30645035
9983  Imaizumi Y, Goda T, Schaffhauser DF, Okada JI, Matsumoto A, Miyahara Y.  Proton-sensing transistor systems for detecting ion leakage from plasma membranes under chemical stimuli.  Acta Biomater  2017  50:502-509  PubMed ID: 27956364
9988  Li S, Gao A, Dong S, Chen Y, Sun S, Lei Z, Zhang Z.  Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.  Int. J. Biol. Macromol.  2017  96:26-34  PubMed ID: 27939510
9792  Hyodo T, Ito S, Asano-Inami E, Chen D, Senga T.  A regulatory subunit of protein phosphatase 2A, PPP2R5E, regulates the abundance of microtubule crosslinking factor 1.  FEBS J.  2016  283:3662-3671  PubMed ID: 27521566



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