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Cell No. : Cell Name
RCB3602 : SDRL-2  update : 2023/10/16
CommentRat cell line derived from liver lacking growth hormone.
Comment from the depositor
Terms and conditions1) As a rule, it make joint researches and required written permission from the approver. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Hum Cell 2010;23:164-172) designated by the DEPOSITOR is required. 3) Before apply for a patent, contact a person who establish a cell line.
Remarks
approver's address
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English
Address
Nippon Medical School, Musashi Kosugi Hospital
Department of Endocrinology, Diabetes and Arteriosclerosis
1-396 Kosugi-machi, Nakahara-ku, Kawasaki-shi, Kanagawa 211-8533 Japan
Dr.ISHIKAWA Mayumi
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishikawa, Mayumi
Originator Ishikawa, Mayumi
Year of deposit 2011
Animal _rat < Mammals
Strain name SDR
Tissue liver
Classification normal
Lifespan finite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_A6JD
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3641  Ishikawa M, Tachibana T, Yoshino G, Hashimoto H, Tanaka T.  Novel functional hepatocyte cell line derived from spontaneous dwarf rat: model of growth hormone function in vitro.  Hum Cell  2010  23(4):164-72  PubMed ID: 21166888   DOI: 10.1111/j.1749-0774.2010.00097.x

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