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RCB2979 : HCT116  update : 2018/07/06
細胞特性(Comment:英)Human cell line derived from colon cancer. Loss of the DNA mismatch repair gene (hMLH1).
細胞特性(日)ヒト大腸癌由来細胞株。ミスマッチ修復遺伝子(hMLH1)欠損。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)Basically, there is no restriction regarding academic use.
使用条件(日)学術研究における利用につきましては原則として使用制限はありません。
備考(英)
備考(日)
提供申込書類 依頼書C-0001.pdf   同意書(非営利)C-0003.pdf   同意書(営利)C-0003p.pdf  
提供同意書は、使用機関及び使用内容に応じて二種類あります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は別途ご相談(cellbank.brc@riken.jp)を承ります。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Shiomi, Tadahiro
寄託日 2009
動物種 human < Mammals
属名 Homo
種名 sapiens
性別 Male
採取組織 colon
細胞分類 cancer
細胞寿命 infinite
細胞形態 epithelial-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (low glucose) + 10% FBS
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
個体識別検査 OK
動物種PCR OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 36件
利用者成果(日) 0件

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Reference(英)
5492  Long BH, Willson JK, Brattain DE, Musial S, Brattain MG.  Effects of mitomycin on human colon carcinoma cells.  J Natl Cancer Inst  1984  73(4):787-92  PubMed ID: 6434780
5491  Brattain MG, Fine WD, Khaled FM, Thompson J, Brattain DE.  Heterogeneity of malignant cells from a human colonic carcinoma.  Cancer Res  1981  41(5):1751-6  PubMed ID: 7214343

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Reference(日)

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利用者成果(英)
6918  Nagasawa I, Muroi M, Kawatani M, Ohishi T, Ohba SI, Kawada M, Osada H.  Identification of a Small Compound Targeting PKM2-Regulated Signaling Using 2D Gel Electrophoresis-Based Proteome-wide CETSA.  Cell Chem Biol  2020  27(2):186-196  PubMed ID: 31813846
9166  Yuka Nakazawa, Yuichiro Hara, Yasuyoshi Oka, Okiru Komine, Diana van den Heuvel, Chaowan Guo, Yasukazu Daigaku, Mayu Isono, Yuxi He, Mayuko Shimada, Kana Kato, Nan Jia, Satoru Hashimoto, Yuko Kotani, Yuka Miyoshi, Miyako Tanaka, Akira Sobue, Norisato Mitsutake, Takayoshi Suganami, Akio Masuda, Kinji Ohno, Shinichiro Nakada, Tomoji Mashimo, Koji Yamanaka, Martijn S Luijsterburg, Tomoo Ogi   Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair  Cell  2020  180 (6), 1228-1244  PubMed ID: 32142649
9913  Satoshi Inoue, Takumi Tsunoda, Miho Riku, Hideaki Ito, Akihito Inoko, Hideki Murakami, Masahide Ebi, Naotaka Ogasawara, Ira Pastan, Kunio Kasugai, Kenji Kasai, Hiroshi Ikeda, Shingo Inaguma  Diffuse Mesothelin Expression Leads to Worse Prognosis Through Enhanced Cellular Proliferation in Colorectal Cancer  Oncol Lett  2020  19(3):1741-1750  PubMed ID: 32194667
11388  Kitada K, Pu F, Toi M.  Occurrence of senescence-escaping cells in doxorubicin-induced senescence is enhanced by PD0332991, a cyclin-dependent kinase 4/6 inhibitor, in colon cancer HCT116 cells.  Oncol Lett  2019    PubMed ID: 30655877
11359  Shimizu D, Masuda T, Sato K, Tsuruda Y, Otsu H, Kuroda Y, Eguchi H, Kodera Y, Mimori K.  CRMP5-associated GTPase (CRAG) Is a Candidate Driver Gene for Colorectal Cancer Carcinogenesis.  Anticancer Res.  2019    PubMed ID: 30591445
11408  Suzuki N, Niikura R, Ihara S, Hikiba Y, Kinoshita H, Higashishima N, Hayakawa Y, Yamada A, Hirata Y, Nakata R, Okamoto M, Sano M, Kushiyama A, Ichinose M, Woods SL, Worthley D, Iwamoto Y, Koike K.  Alpha-Blockers As Colorectal Cancer Chemopreventive: Findings from a Case-Control Study, Human Cell Cultures, and In Vivo Preclinical Testing.  Cancer Prev Res (Phila)  2019    PubMed ID: 30700439
11517  Sekido M, Fujita KI, Kubota Y, Ishida H, Takahashi T, Ohkuma R, Tsunoda T, Ishikawa F, Shibanuma M, Sasaki Y.  Rabeprazole intake does not affect systemic exposure to capecitabine and its metabolites, 5'-deoxy-5-fluorocytidine, 5'-deoxy-5-fluorouridine, and 5-fluorouracil.  Cancer Chemother. Pharmacol.  2019    PubMed ID: 30972456
11725  Kawasaki Y, Miyamoto M, Oda T, Matsumura K, Negishi L, Nakato R, Suda S, Yokota N, Shirahige K, Akiyama T.  The novel lncRNA CALIC upregulates AXL to promote colon cancer metastasis.  EMBO Rep.  2019    PubMed ID: 31353791
11835  Yokoyama Y, Sakatani T, Wada R, Ishino K, Kudo M, Koizumi M, Yamada T, Yoshida H, Naito Z.  In vitro and in vivo studies on the association of long non‑coding RNAs H19 and urothelial cancer associated 1 with the susceptibility to 5‑fluorouracil in rectal cancer.  Int. J. Oncol.  2019    PubMed ID: 31638183
11851  Fujitani N, Yoneda A, Takahashi M, Takasawa A, Aoyama T, Miyazaki T.  Silencing of Glutathione S-Transferase Pi Inhibits Cancer Cell Growth via Oxidative Stress Induced by Mitochondria Dysfunction.  Sci Rep  2019    PubMed ID: 31611630
11864  Matsumoto Y, Kuriki H, Kitamura T, Takahashi D, Toshima K.  Total Synthesis and Structure-Activity Relationship Study of Vineomycin A1.  J. Org. Chem.  2019    PubMed ID: 31642324
9501  Shiota M, Naya M, Yamamoto T, Hishiki T, Tani T, Takahashi H, Kubo A, Koike D, Itoh M, Ohmura M, Kabe Y, Sugiura Y, Hiraoka N, Morikawa T, Takubo K, Suina K, Nagashima H, Sampetrean O, Nagano O, Saya H, Yamazoe S, Watanabe H, Suematsu M.  Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival.  Nat Commun  2018  9:1561  PubMed ID: 29674746
10580  Mito A, Nakano Y, Saitoh T, Gouraud SSS, Yamaguchi Y, Sato T, Sasaki N, Kojima-Aikawa K.  Lectin ZG16p inhibits proliferation of human colorectal cancer cells via its carbohydrate-binding sites.  Glycobiology  2018  28:21-31  PubMed ID: 29069492
10606  Kanno E, Kawasaki O, Takahashi K, Takano K, Endo T.  DA-Raf, a dominant-negative antagonist of the Ras-ERK pathway, is a putative tumor suppressor.  Exp. Cell Res.  2018  362:111-120  PubMed ID: 29129563
10702  Horibe S, Tanahashi T, Kawauchi S, Murakami Y, Rikitake Y.  Mechanism of recipient cell-dependent differences in exosome uptake.  BMC Cancer  2018  18:47  PubMed ID: 29306323
10751  Noguchi A, Adachi S, Yokota N, Hatta T, Natsume T, Kawahara H.  ZFP36L2 is a cell cycle-regulated CCCH protein necessary for DNA lesion-induced S-phase arrest.  Biol Open  2018  7:  PubMed ID: 29449217
10757  Seiler M, Yoshimi A, Darman R, Chan B, Keaney G, Thomas M, Agrawal AA, Caleb B, Csibi A, Sean E, Fekkes P, Karr C, Klimek V, Lai G, Lee L, Kumar P, Lee SC, Liu X, Mackenzie C, Meeske C, Mizui Y, Padron E, Park E, Pazolli E, Peng S, Prajapati S, Taylor J, Teng T, Wang J, Warmuth M, Yao H, Yu L, Zhu P, Abdel-Wahab O, Smith PG, Buonamici S.  H3B-8800, an orally available small-molecule splicing modulator, induces lethality in spliceosome-mutant cancers.  Nat. Med.  2018  24:497-504  PubMed ID: 29457796
10847  Kojima K, Nakamura T, Ohbu M, Katoh H, Ooizumi Y, Igarashi K, Ishii S, Tanaka T, Yokoi K, Nishizawa N, Yokota K, Kosaka Y, Sato T, Watanabe M, Yamashita K.  Cysteine dioxygenase type 1 (CDO1) gene promoter methylation during the adenoma-carcinoma sequence in colorectal cancer.  PLoS ONE  2018  13:e0194785  PubMed ID: 29746493
11009    Evidence suggesting sphingosine 1-phosphate may be actively generated, degraded and transported to extracellular spaces with increased S1P2 and S1P3 expressions in colon cancer  Clinical Colorectal Cancer  2018  17, e171-e182  PubMed ID: 29223361
11087  Thar Min AK, Okayama H, Saito M, Ashizawa M, Aoto K, Nakajima T, Saito K, Hayase S, Sakamoto W, Tada T, Hanayama H, Saze Z, Momma T, Ohki S, Sato Y, Motoyama S, Mimura K, Kono K.  Epithelial-mesenchymal transition-converted tumor cells can induce T-cell apoptosis through upregulation of programmed death ligand 1 expression in esophageal squamous cell carcinoma.  Cancer Med  2018    PubMed ID: 29855157
4422  Enos Tangke Arung, Rudianto Amirta, Qinchang Zhu, Yhiya Amen, Kuniyoshi Shimizu  Effect of wood, bark and leaf extracts of Macaranga trees on cytotoxic activity in some cancer and normal cell lines  Journal of the Indian Academy of Wood Science  2018  5, 115–119 
4195  Ishigaki Y, Sato K.  Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions.  Micromachines (Basel)  2018    PubMed ID: 30424205
4196  Wada F, Koga H, Akiba J, Niizeki T, Iwamoto H, Ikezono Y, Nakamura T, Abe M, Masuda A, Sakaue T, Tanaka T, Kakuma T, Yano H, Torimura T.  High expression of CD44v9 and xCT in chemoresistant hepatocellular carcinoma: Potential targets by sulfasalazine.  Cancer Sci.  2018    PubMed ID: 29981246
4344  Li HK, Hasegawa S, Nakajima NI, Morokoshi Y, Minegishi K, Nagatsu K.  Targeted cancer cell ablation in mice by an α-particle-emitting astatine-211-labeled antibody against major histocompatibility complex class I chain-related protein A and B.  Biochem. Biophys. Res. Commun.  2018    PubMed ID: 30409432
9847  Koike M, Yutoku Y, Koike A.  Cloning, localization and focus formation at DNA damage sites of canine XRCC4.  J. Vet. Med. Sci.  2017  78:1865-1871  PubMed ID: 27644316
10185  Yokoi K, Yamashita K, Ishii S, Tanaka T, Nishizawa N, Tsutsui A, Miura H, Katoh H, Yamanashi T, Naito M, Sato T, Nakamura T, Watanabe M.  Comprehensive molecular exploration identified promoter DNA methylation of the CRBP1 gene as a determinant of radiation sensitivity in rectal cancer.  Br. J. Cancer  2017  116:1046-1056  PubMed ID: 28291773
10296  Gombodorj N, Yokobori T, Yoshiyama S, Kawabata-Iwakawa R, Rokudai S, Horikoshi I, Nishiyama M, Nakano T.  Inhibition of Ubiquitin-conjugating Enzyme E2 May Activate the Degradation of Hypoxia-inducible Factors and, thus, Overcome Cellular Resistance to Radiation in Colorectal Cancer.  Anticancer Res.  2017  37:2425-2436  PubMed ID: 28476810
10498  Inaguma S, Lasota J, Felisiak-Golabek A, Kowalik A, Wang Z, Zieba S, Kalisz J, Ikeda H, Miettinen M.  Histopathological and genotypic characterization of metastatic colorectal carcinoma with PD-L1 (CD274)-expression: Possible roles of tumour micro environmental factors for CD274 expression.  J Pathol Clin Res  2017  3:268-278  PubMed ID: 29085667
10520  Koike M, Yutoku Y, Koike A.  Cloning of canine Ku80 and its localization and accumulation at DNA damage sites.  FEBS Open Bio  2017  7:1854-1863  PubMed ID: 29226073
10535  Ohashi W, Yamamine N, Imura J, Hattori Y.  SKL2001 suppresses colon cancer spheroid growth through regulation of the E-cadherin/β-Catenin complex.  Biochem. Biophys. Res. Commun.  2017  493:1342-1348  PubMed ID: 28970068
4194  Satoh T, Sugiura S, Shin K, Onuki-Nagasaki R, Ishida S, Kikuchi K, Kakiki M, Kanamori T.  A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform.  Lab Chip  2017    PubMed ID: 29184959
10028  Michinori Takashina, Sayaka Inoue, Kei Tomihara, Kengo Tomita, Kohshi Hattori, Qing-Li Zhao, Tokiko Suzuki, Makoto Noguchi, Wakana Ohashi, Yuichi Hattori  Different Effect of Resveratrol to Induction of Apoptosis Depending on the Type of Human Cancer Cells  Int J Oncol  2017  50(3):787-797.  PubMed ID: 28197625
10913    Clinical potential of DAC-70 sol  Integr Mol Med  2016  3: 645-648 
7305  Yamada K, Maishi N, Akiyama K, Towfik Alam M, Ohga N, Kawamoto T, Shindoh M, Takahashi N, Kamiyama T, Hida Y, Taketomi A, Hida K.  CXCL12-CXCR7 axis is important for tumor endothelial cell angiogenic property.  Int. J. Cancer  2015  137:2825-36  PubMed ID: 26100110
7560  Shimodaira S, Hirabayashi K, Koya T, Higuchi Y, Sano K  Standard WT1 Expression Using Immunohistochemistry.  J Mol Biomark Diagn  2015  7:266 
4397  Pan J, Zhong J, Gan LH, Chen SJ, Jin HC, Wang X, Wang LJ.  Klotho, an anti-senescence related gene, is frequently inactivated through promoter hypermethylation in colorectal cancer.  Tumour Biol  2011  32(4):729-35  PubMed ID: 21523445

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