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RCB2979 : HCT116  update : 2018/07/06
CommentHuman cell line derived from colon cancer. Loss of the DNA mismatch repair gene (hMLH1).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Shiomi, Tadahiro
Year of deposit 2009
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue colon
Classification cancer
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Animal PCR OK
deposit info
lot info
Reference information Reference 2
User's Publication 33

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5491  Heterogeneity of malignant cells from a human colonic carcinoma.  Brattain MG, Fine WD, Khaled FM, Thompson J, Brattain DE.  Cancer Res  1981  41(5):1751-6  PubMed ID: 7214343
5492  Effects of mitomycin on human colon carcinoma cells.  Long BH, Willson JK, Brattain DE, Musial S, Brattain MG.  J Natl Cancer Inst  1984  73(4):787-92  PubMed ID: 6434780

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User's Publication
7305  CXCL12-CXCR7 axis is important for tumor endothelial cell angiogenic property.  Yamada K, Maishi N, Akiyama K, Towfik Alam M, Ohga N, Kawamoto T, Shindoh M, Takahashi N, Kamiyama T, Hida Y, Taketomi A, Hida K.  Int. J. Cancer  2015  137:2825-36  PubMed ID: 26100110
7560  Standard WT1 Expression Using Immunohistochemistry.  Shimodaira S, Hirabayashi K, Koya T, Higuchi Y, Sano K  J Mol Biomark Diagn  2015  7:266 
9501  Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival.  Shiota M, Naya M, Yamamoto T, Hishiki T, Tani T, Takahashi H, Kubo A, Koike D, Itoh M, Ohmura M, Kabe Y, Sugiura Y, Hiraoka N, Morikawa T, Takubo K, Suina K, Nagashima H, Sampetrean O, Nagano O, Saya H, Yamazoe S, Watanabe H, Suematsu M.  Nat Commun  2018  9:1561  PubMed ID: 29674746
9847  Cloning, localization and focus formation at DNA damage sites of canine XRCC4.  Koike M, Yutoku Y, Koike A.  J. Vet. Med. Sci.  2017  78:1865-1871  PubMed ID: 27644316
10185  Comprehensive molecular exploration identified promoter DNA methylation of the CRBP1 gene as a determinant of radiation sensitivity in rectal cancer.  Yokoi K, Yamashita K, Ishii S, Tanaka T, Nishizawa N, Tsutsui A, Miura H, Katoh H, Yamanashi T, Naito M, Sato T, Nakamura T, Watanabe M.  Br. J. Cancer  2017  116:1046-1056  PubMed ID: 28291773
10296  Inhibition of Ubiquitin-conjugating Enzyme E2 May Activate the Degradation of Hypoxia-inducible Factors and, thus, Overcome Cellular Resistance to Radiation in Colorectal Cancer.  Gombodorj N, Yokobori T, Yoshiyama S, Kawabata-Iwakawa R, Rokudai S, Horikoshi I, Nishiyama M, Nakano T.  Anticancer Res.  2017  37:2425-2436  PubMed ID: 28476810
10498  Histopathological and genotypic characterization of metastatic colorectal carcinoma with PD-L1 (CD274)-expression: Possible roles of tumour micro environmental factors for CD274 expression.  Inaguma S, Lasota J, Felisiak-Golabek A, Kowalik A, Wang Z, Zieba S, Kalisz J, Ikeda H, Miettinen M.  J Pathol Clin Res  2017  3:268-278  PubMed ID: 29085667
10520  Cloning of canine Ku80 and its localization and accumulation at DNA damage sites.  Koike M, Yutoku Y, Koike A.  FEBS Open Bio  2017  7:1854-1863  PubMed ID: 29226073
10535  SKL2001 suppresses colon cancer spheroid growth through regulation of the E-cadherin/β-Catenin complex.  Ohashi W, Yamamine N, Imura J, Hattori Y.  Biochem. Biophys. Res. Commun.  2017  493:1342-1348  PubMed ID: 28970068
10580  Lectin ZG16p inhibits proliferation of human colorectal cancer cells via its carbohydrate-binding sites.  Mito A, Nakano Y, Saitoh T, Gouraud SSS, Yamaguchi Y, Sato T, Sasaki N, Kojima-Aikawa K.  Glycobiology  2018  28:21-31  PubMed ID: 29069492
10606  DA-Raf, a dominant-negative antagonist of the Ras-ERK pathway, is a putative tumor suppressor.  Kanno E, Kawasaki O, Takahashi K, Takano K, Endo T.  Exp. Cell Res.  2018  362:111-120  PubMed ID: 29129563
10702  Mechanism of recipient cell-dependent differences in exosome uptake.  Horibe S, Tanahashi T, Kawauchi S, Murakami Y, Rikitake Y.  BMC Cancer  2018  18:47  PubMed ID: 29306323
10751  ZFP36L2 is a cell cycle-regulated CCCH protein necessary for DNA lesion-induced S-phase arrest.  Noguchi A, Adachi S, Yokota N, Hatta T, Natsume T, Kawahara H.  Biol Open  2018  7:  PubMed ID: 29449217
10757  H3B-8800, an orally available small-molecule splicing modulator, induces lethality in spliceosome-mutant cancers.  Seiler M, Yoshimi A, Darman R, Chan B, Keaney G, Thomas M, Agrawal AA, Caleb B, Csibi A, Sean E, Fekkes P, Karr C, Klimek V, Lai G, Lee L, Kumar P, Lee SC, Liu X, Mackenzie C, Meeske C, Mizui Y, Padron E, Park E, Pazolli E, Peng S, Prajapati S, Taylor J, Teng T, Wang J, Warmuth M, Yao H, Yu L, Zhu P, Abdel-Wahab O, Smith PG, Buonamici S.  Nat. Med.  2018  24:497-504  PubMed ID: 29457796
10847  Cysteine dioxygenase type 1 (CDO1) gene promoter methylation during the adenoma-carcinoma sequence in colorectal cancer.  Kojima K, Nakamura T, Ohbu M, Katoh H, Ooizumi Y, Igarashi K, Ishii S, Tanaka T, Yokoi K, Nishizawa N, Yokota K, Kosaka Y, Sato T, Watanabe M, Yamashita K.  PLoS ONE  2018  13:e0194785  PubMed ID: 29746493
10913  Clinical potential of DAC-70 sol    Integr Mol Med  2016  3: 645-648 
11009  Evidence suggesting sphingosine 1-phosphate may be actively generated, degraded and transported to extracellular spaces with increased S1P2 and S1P3 expressions in colon cancer    Clinical Colorectal Cancer  2018  17, e171-e182  PubMed ID: 29223361
11087  Epithelial-mesenchymal transition-converted tumor cells can induce T-cell apoptosis through upregulation of programmed death ligand 1 expression in esophageal squamous cell carcinoma.  Thar Min AK, Okayama H, Saito M, Ashizawa M, Aoto K, Nakajima T, Saito K, Hayase S, Sakamoto W, Tada T, Hanayama H, Saze Z, Momma T, Ohki S, Sato Y, Motoyama S, Mimura K, Kono K.  Cancer Med  2018    PubMed ID: 29855157
11388  Occurrence of senescence-escaping cells in doxorubicin-induced senescence is enhanced by PD0332991, a cyclin-dependent kinase 4/6 inhibitor, in colon cancer HCT116 cells.  Kitada K, Pu F, Toi M.  Oncol Lett  2019    PubMed ID: 30655877
11359  CRMP5-associated GTPase (CRAG) Is a Candidate Driver Gene for Colorectal Cancer Carcinogenesis.  Shimizu D, Masuda T, Sato K, Tsuruda Y, Otsu H, Kuroda Y, Eguchi H, Kodera Y, Mimori K.  Anticancer Res.  2019    PubMed ID: 30591445
11408  Alpha-Blockers As Colorectal Cancer Chemopreventive: Findings from a Case-Control Study, Human Cell Cultures, and In Vivo Preclinical Testing.  Suzuki N, Niikura R, Ihara S, Hikiba Y, Kinoshita H, Higashishima N, Hayakawa Y, Yamada A, Hirata Y, Nakata R, Okamoto M, Sano M, Kushiyama A, Ichinose M, Woods SL, Worthley D, Iwamoto Y, Koike K.  Cancer Prev Res (Phila)  2019    PubMed ID: 30700439
4422  Effect of wood, bark and leaf extracts of Macaranga trees on cytotoxic activity in some cancer and normal cell lines  Enos Tangke Arung, Rudianto Amirta, Qinchang Zhu, Yhiya Amen, Kuniyoshi Shimizu  Journal of the Indian Academy of Wood Science  2018  5, 115–119 
4194  A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform.  Satoh T, Sugiura S, Shin K, Onuki-Nagasaki R, Ishida S, Kikuchi K, Kakiki M, Kanamori T.  Lab Chip  2017    PubMed ID: 29184959
4195  Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions.  Ishigaki Y, Sato K.  Micromachines (Basel)  2018    PubMed ID: 30424205
4196  High expression of CD44v9 and xCT in chemoresistant hepatocellular carcinoma: Potential targets by sulfasalazine.  Wada F, Koga H, Akiba J, Niizeki T, Iwamoto H, Ikezono Y, Nakamura T, Abe M, Masuda A, Sakaue T, Tanaka T, Kakuma T, Yano H, Torimura T.  Cancer Sci.  2018    PubMed ID: 29981246
4344  Targeted cancer cell ablation in mice by an α-particle-emitting astatine-211-labeled antibody against major histocompatibility complex class I chain-related protein A and B.  Li HK, Hasegawa S, Nakajima NI, Morokoshi Y, Minegishi K, Nagatsu K.  Biochem. Biophys. Res. Commun.  2018    PubMed ID: 30409432
4397  Klotho, an anti-senescence related gene, is frequently inactivated through promoter hypermethylation in colorectal cancer.  Pan J, Zhong J, Gan LH, Chen SJ, Jin HC, Wang X, Wang LJ.  Tumour Biol  2011  32(4):729-35  PubMed ID: 21523445
11517  Rabeprazole intake does not affect systemic exposure to capecitabine and its metabolites, 5'-deoxy-5-fluorocytidine, 5'-deoxy-5-fluorouridine, and 5-fluorouracil.  Sekido M, Fujita KI, Kubota Y, Ishida H, Takahashi T, Ohkuma R, Tsunoda T, Ishikawa F, Shibanuma M, Sasaki Y.  Cancer Chemother. Pharmacol.  2019    PubMed ID: 30972456
11725  The novel lncRNA CALIC upregulates AXL to promote colon cancer metastasis.  Kawasaki Y, Miyamoto M, Oda T, Matsumura K, Negishi L, Nakato R, Suda S, Yokota N, Shirahige K, Akiyama T.  EMBO Rep.  2019    PubMed ID: 31353791
11835  In vitro and in vivo studies on the association of long non‑coding RNAs H19 and urothelial cancer associated 1 with the susceptibility to 5‑fluorouracil in rectal cancer.  Yokoyama Y, Sakatani T, Wada R, Ishino K, Kudo M, Koizumi M, Yamada T, Yoshida H, Naito Z.  Int. J. Oncol.  2019    PubMed ID: 31638183
11851  Silencing of Glutathione S-Transferase Pi Inhibits Cancer Cell Growth via Oxidative Stress Induced by Mitochondria Dysfunction.  Fujitani N, Yoneda A, Takahashi M, Takasawa A, Aoyama T, Miyazaki T.  Sci Rep  2019    PubMed ID: 31611630
11864  Total Synthesis and Structure-Activity Relationship Study of Vineomycin A1.  Matsumoto Y, Kuriki H, Kitamura T, Takahashi D, Toshima K.  J. Org. Chem.  2019    PubMed ID: 31642324
6918  Identification of a Small Compound Targeting PKM2-Regulated Signaling Using 2D Gel Electrophoresis-Based Proteome-wide CETSA.  Nagasawa I, Muroi M, Kawatani M, Ohishi T, Ohba SI, Kawada M, Osada H.  Cell Chem Biol  2019    PubMed ID: 31813846

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