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Cell No. : Cell Name
RCB2945 : DIT29  update : 2023/10/16
CommentMedaka cell line derived from hepatoma cells.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE is provided as a service to research community. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Fac Sci Univ Tokyo IV 1980;14:391-398 ) designated by the DEPOSITOR is required. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mitani, Hiroshi
Originator Mitani, Hiroshi
Year of deposit 2009
Animal _medaka < Fishes
Genus Oryzias
Species latipes
Tissue liver
Disease name hepatoma
Classification cancer
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_J028
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium L-15 + 10mM HEPES + 20% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 6-8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Images
deposit info
lot info
Reference information Reference 1
User's Publication 3


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Reference
3635      J Fac Sci Univ Tokyo IV  1980  14:391-398   

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User's Publication
20676  Tran NT, Vo LK, Komatsu M, Shiozaki K.  Involvement of N-acetylneuraminate cytidylyltransferase in Edwardsiella piscicida pathogenicity.  Fish Shellfish Immunol  2022  124:534-542  PubMed ID: 35477099   DOI: 10.1016/j.fsi.2022.04.033
15275  Oishi K, Morise M, Vo LK, Tran NT, Sahashi D, Ueda-Wakamatsu R, Nishimura W, Komatsu M, Shiozaki K.  Host lactosylceramide enhances Edwardsiella tarda infection  Cell Microbiol  2021  23(9):e13365  PubMed ID: 33988901   DOI: 10.1111/cmi.13365
12624  Maekawa S, Chiang YA, Hikima J, Sakai M, Lo CF, Wang HC, Aoki T.  Expression and biological activity of two types of interferon genes in medaka (Oryzias latipes)  Fish Shellfish Immunol  2016  48:20-9  PubMed ID: 26655956   DOI: 10.1016/j.fsi.2015.11.036



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