Cell No. : Cell Name RCB2911 : MEDEP-BRC5  update : 2024/01/17
Comment			Mouse erythroid cell line able to differentiate into enucleated red blood cells. Derived from mouse ES cell line, BRC5 (AES0009). SCF(Stem cell factor) dependent.
Comment from the depositor
Terms and conditions			In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS ONE 2008;3:e1544) designated by the DEPOSITOR is required.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Hiroyama, Takashi
		Originator	Hiroyama, Takashi
		Year of deposit	2009
		Another name	B6EP-5(S)
		Animal	_mouse < Mammals
		Strain name	C57BL/6
		Gender	Male
		Age at sampling	embryo
		Tissue	embryonic stem cells : BRC5(AES009)
		Classification	other
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_E137
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture medium		IMDM (without L-Glutamine) + 2mM L-Glutamine + 15% FBS + ITS (SIGMA #I3146) + 0.45mM α-monothioglycerol + 10 -6M Dexamethasone + 50μg/ml Ascorbic acid + 50ng/ml mouse SCF
		Culture method
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 3 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
		Others		Thawing of frozen cells : spin at 1200rpm 3min.
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	3

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Reference

8770  Hiroyama T, Miharada K, Sudo K, Danjo I, Aoki N, Nakamura Y.  Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells.  PLoS ONE  2008  3:e1544  PubMed ID: 18253492   DOI: 10.1371/journal.pone.0001544

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User's Publication

13018  Kiatpakdee B, Sato K, Otsuka Y, Arashiki N, Chen Y, Tsumita T, Otsu W, Yamamoto A, Kawata R, Yamazaki J, Sugimoto Y, Takada K, Mohandas N, Inaba M.  Cholesterol-binding protein TSPO2 coordinates maturation and proliferation of terminally differentiating erythroblasts.  J Biol Chem  2020    PubMed ID: 32358067   DOI: 10.1074/jbc.RA119.011679

11390  Yang C, Hashimoto M, Lin QXX, Tan DQ, Suda T.  Sphingosine-1-phosphate signaling modulates terminal erythroid differentiation through the regulation of mitophagy.  Exp. Hematol.  2019    PubMed ID: 30658118   DOI: 10.1016/j.exphem.2019.01.004

8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014    DOI: 10.4051/ibc.2010.2.4.0014