Cell No. : Cell Name RCB2909 : Walker256-TC  update : 2023/10/16
Comment			Rat cell line derived from mammary carcinoma. TKG0565 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Fukuda, Hiroshi
		Year of deposit	2008
		Original cell	TKG0565
		Animal	_rat < Mammals
		Strain name	Wistar
		Gender	Female
		Tissue	mammary gland
		Disease name	mammary gland carcinoma
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0565）
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_4984
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent/Suspension cells
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 6 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP, AST
Images			deposit info	lot info
Reference information		Reference	6
		User's Publication	1

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Reference

5388  Arakawa M, Shimizu F, Sasagawa K, Inomata T, Shinkai K.  Antitumor activity of methyl (5-fluoro-1H-2-oxo-4-pyrimidinyl beta-D-glucopyranosid)uronate against various experimental tumors.  Gan  1981  72(2):220-5  PubMed ID: 6793441

5389  Chen CH, Chen SC.  Cell growth factor activity. New quantitative method in cell culture assay.  Exp Cell Res  1981  136(1):43-51  PubMed ID: 7028494   DOI: 10.1016/0014-4827(81)90036-7

5390  Wass JA, Varani J, Piontek GE, Goff D, Ward PA.  Characteristics of the chemotactic factor-mediated cell swelling response of tumor cells.  J Natl Cancer Inst  1981  66(5):927-33  PubMed ID: 6939935

5391  Goodlad GA, Clark CM.  Actin polymerisation in Walker 256 carcinoma cells from solid or ascitic tumours.  Cancer Lett  1981  13(2):129-32  PubMed ID: 7306949   DOI: 10.1016/0304-3835(81)90138-5

5387  DUNHAM LJ, STEWART HL.  A survey of transplantable and transmissible animal tumors.  J Natl Cancer Inst  1953  13:1299-377  PubMed ID: 13035452

5386  WILTON R. EARLE   A study of the Walker rat mammary carcinoma 256, in vivo and in vitro  Am J Cancer  1935  24, 566-612    DOI: 10.1158/ajc.1935.566

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User's Publication

13438  Takayuki Kamanaka, Hisao Haniu, Manabu Tanaka, Takashi Takizawa, Kaoru Aoki, Masanori Okamoto, Atsushi Sobajima, Kazushige Yoshida, Hirokazu Ideta, Tetsuhiko Mimura, Haruka Ishida, Katsuya Ueda, Takeshi Uemura, Jin Hee Kim, Yoong Ahm Kim, Hiroyuki Katoa and Naoto Saito  Carbon fibers for treatment of cancer metastasis in bone  RSC Adv  2020    PubMed ID: 35515018   DOI: 10.1039/d0ra05992g