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RCB2869 : CHO-K1  update : 2018/08/02
CommentA subline of CHO cells. Require proline. TKG0328 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Puck, T. T.
Year of deposit 2008
Original cell TKG0328
Animal hamster < Mammals
Strain name Chinese
Tissue ovary
Classification mutant
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0328)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
10729  Asano K, Suzuki T, Saito A, Wei FY, Ikeuchi Y, Numata T, Tanaka R, Yamane Y, Yamamoto T, Goto T, Kishita Y, Murayama K, Ohtake A, Okazaki Y, Tomizawa K, Sakaguchi Y, Suzuki T.  Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease.  Nucleic Acids Res.  2018  46:1565-1583  PubMed ID: 29390138



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