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Cell No. : Cell Name
RCB2869 : CHO-K1  update : 2024/03/21
CommentA subline of CHO cells. Require proline. TKG0328 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Puck, T. T.
Year of deposit 2008
Original cell TKG0328
Animal _hamster < Mammals
Strain name Chinese
Tissue ovary
Classification mutant
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0328)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0214
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
Images
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Reference information Reference 0
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