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RCB2867 : HeLa/Fucci2  update : 2019/02/27
CommentA subline of the HeLa cell line expressing Fucci2, a cell cycle marker. Cell line name : HeLa/Fucci2 #3. Important notice regarding nomenclature of Fucci.
Comment from the depositor
Terms and conditions1) The RECIPIENT should obtain written approval from the UCSD using the [mCherry FORM]. 2) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3) In publishing the research results related to the BIOLOGICAL RESOURCE,a citation of the following literature is necessary. Asako Sakaue-Sawano, Tamiyo Kobayashi, Kenji Ohtawa and Atsushi Miyawaki. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol.12:2. 4) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Miyawaki, Atsushi
Originator Miyawaki, Atsushi
Year of deposit 2009
Another name HeLa.S-Fucci2
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Case history cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene Fucci2
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 4


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Reference
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265 
7987  Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A.  Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.  BMC Cell Biol.  2011  12:2  PubMed ID: 21226962

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User's Publication
7021  Sasaki T, Sato Y, Higashiyama T, Sasaki N.  Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells.  Sci Rep  2017  7:11257  PubMed ID: 28900194
10632  Tsuda Y, Iimori M, Nakashima Y, Nakanishi R, Ando K, Ohgaki K, Kitao H, Saeki H, Oki E, Maehara Y.  Mitotic slippage and the subsequent cell fates after inhibition of Aurora B during tubulin-binding agent-induced mitotic arrest.  Sci Rep  2017  7:16762  PubMed ID: 29196757
10910    Intracellular calcium dynamics and expression of P2Y and IP3 receptors in a cycling G1-phase cell  Bioimages  2016  24 : 13-29 
4211  Panagiotakopoulou M, Lendenmann T, Pramotton FM, Giampietro C, Stefopoulos G, Poulikakos D, Ferrari A.  Cell cycle-dependent force transmission in cancer cells.  Mol. Biol. Cell  2018    PubMed ID: 30113874



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