細胞番号 : 細胞名 RCB2854 : C6  update : 2024/01/05
細胞特性(Comment:英)			Rat cell line derived from glial cell tumor induced by N-nitrosomethylurea. TKG0589 (Deposited from Tohoku Univ.).
細胞特性(日)			ラット神経グリア細胞腫瘍に由来する細胞株。N-nitrosomethylureaによって誘発した腫瘍。 TKG0589 (東北大学医用細胞資源センターからの寄託)。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)			基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Fukuda, Hiroshi
		寄託日	2008
		元の細胞	TKG0589
		動物種	_rat < Mammals
		採取組織	brain
		病名	glial cell tumor
		細胞分類	cancer
		入手歴	Cell Resource Center for Biomedical Research, Tohoku University（TKG0589）
		細胞寿命	infinite
		細胞形態	other
		Cellosaurus(Expasy)	CVCL_0194
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		RPMI1640 + 10% FBS
		抗生物質		Free
		継代方法		0.25% Trypsin
		継代密度		1 : 8 split
		継代・培地交換頻度		Subculture : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ		(-)
		動物種PCR		OK
		アイソザイム検査		LD, NP, AST, PepB
画像情報			寄託時情報	ロット情報
文献情報		Reference(英)	6件
		Reference(日)	0件
		利用者成果(英)	22件
		利用者成果(日)	0件

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Reference(英)

5354  Zagyansky Y, Benda P, Bisconte JC.  Restricted lateral diffusion of concanavalin A receptors of different malignant cells of the nervous system.  FEBS Lett  1977  77(2):206-8  PubMed ID: 862919   DOI: 10.1016/0014-5793(77)80235-4

5353  Rieger F, Faivre-Bauman A, Benda P, Vigny M.  Molecular forms of acetylcholinesterase: their de novo synthesis in mouse neuroblastoma cells.  J Neurochem  1976  27(5):1059-63  PubMed ID: 12170588   DOI: 10.1111/j.1471-4159.1976.tb00308.x

5352  Premont J, Benda P, Jard S.  [3H] norepinephrine binding by rat glial cells in culture. Lack of correlation between binding and adenylate cyclase activation.  Biochim Biophys Acta  1975  381(2):368-76  PubMed ID: 163103   DOI: 10.1016/0304-4165(75)90242-1

5351  Penit J, Jard S, Benda P.  Probenecide sensitive 3'-5'-cyclic AMP secretion by isoproterenol stimulated glial cells in culture.  FEBS Lett  1974  41(1):156-60  PubMed ID: 4369045   DOI: 10.1016/0014-5793(74)80977-4

5350  Jard S, Premont J, Benda P.  Adenylate cyclase, phosphodiesterases and protein kinase of rat glial cells in culture.  FEBS Lett  1972  26(1):344-8  PubMed ID: 4344295   DOI: 10.1016/0014-5793(72)80608-2

645  Benda P, Lightbody J, Sato G, Levine L, Sweet W.  Differentiated rat glial cell strain in tissue culture.  Science  1968  161(3839):370-1  PubMed ID: 4873531   DOI: 10.1126/science.161.3839.370

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Reference(日)

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利用者成果(英)

21576  Naima J, Ohta Y.  Potassium Ions Decrease Mitochondrial Matrix pH: Implications for ATP Production and Reactive Oxygen Species Generation.  Int J Mol Sci  2024  25(2)  PubMed ID: 38279231   DOI: 10.3390/ijms25021233

21623  Kaizuka Y, Suzuki H, Watabe T, Ooe K, Toyoshima A, Takahashi K, Sawada K, Iimori T, Masuda Y, Uno T, Kannaka K, Uehara T.  Neopentyl glycol-based radiohalogen-labeled amino acid derivatives for cancer radiotheranostics.  EJNMMI Radiopharm Chem  2024  9(1):17  PubMed ID: 38407647   DOI: 10.1186/s41181-024-00244-4

21036  Sato K, Suzuki-Utsunomiya K, Mitsui S, Ono S, Shimakura K, Otomo A, Hadano S.  Central nervous system specific high molecular weight ALS2/alsin homophilic complex is enriched in mouse brain synaptosomes.  Biochem Biophys Res Commun  2023  638:168-175  PubMed ID: 36459881   DOI: 10.1016/j.bbrc.2022.11.061

17503  Cui M, Takahashi M, Chen Y, Liu B, Ohta Y, Miyake T.  pH modulation in adhesive cells with a protonic biotransducer  Bioelectrochemistry  2022  147:108202  PubMed ID: 35810497   DOI: 10.1016/j.bioelechem.2022.108202

17864  Fukuda S, Akiyama M, Niki Y, Kawatsura R, Harada H, Nakahama KI.  Inhibitory effects of miRNAs in astrocytes on C6 glioma progression via connexin 43  Mol Cell Biochem  2021  476(7):2623-2632  PubMed ID: 33660186   DOI: 10.1007/s11010-021-04118-0

20426  Mitsuyuki K, Watabe T, Naka S, Liu Y, Tatsumi M, Shimosegawa E, Kato H.  Evaluation of Integrin αvβ3 Expression in Murine Xenograft Models: [68Ga]Ga-DOTA-C(RGDfK) PET Study with Immunohistochemical Confirmation.  Diagnostics (Basel)  2021  11(7)  PubMed ID: 34359378   DOI: 10.3390/diagnostics11071295

6917  Watabe T　et al  Theranostics targeting fibroblast activation protein in the tumor stroma: 64Cu and 225Ac labelled FAPI-04 in pancreatic cancer xenograft mouse models.  J Nucl Med  2020  61(4):563-569  PubMed ID: 31586001   DOI: 10.2967/jnumed.119.233122

12991  Watabe T, Kaneda-Nakashima K, Shirakami Y, Liu Y, Ooe K, Teramoto T, Toyoshima A, Shimosegawa E, Nakano T, Kanai Y, Shinohara A, Hatazawa J.  Targeted alpha therapy using astatine (211At)-labeled phenylalanine: A preclinical study in glioma bearing mice.  Oncotarget  2020    PubMed ID: 32341757   DOI: 10.18632/oncotarget.27552

13601  Hirai N, Watabe T, Nagamori S, Wiriyasermkul P, Tanaka Y, Romanov V, Naka S, Kanai Y, Liu Y, Tani N, Sakai T, Tatsumi M, Shimosegawa E, Kanai Y, Hatazawa J.  Evaluation of D-isomer of 18 F-FBPA for oncology PET focusing on the differentiation of glioma and inflammation  Asia Ocean J Nucl Med Biol  2020  8(2):102-108.  PubMed ID: 32714997   DOI: 10.22038/AOJNMB.2020.47399.1321

5357  Takahashi D, Moriyama J, Nakamura T, Miki E, Takahashi E, Sato A, Akaike T, Itto-Nakama K, Arimoto H.  AUTACs: Cargo-Specific Degraders Using Selective Autophagy.  Mol Cell  2019  76(5):797-810  PubMed ID: 31606272   DOI: 10.1016/j.molcel.2019.09.009

11209  Yamada D, Fujikawa K, Kawabe K, Furuta T, Nakada M, Takarada T.  RUNX2 Promotes Malignant Progression in Glioma.  Neurochem. Res.  2018    PubMed ID: 30203400   DOI: 10.1007/s11064-018-2626-4

9806  Watabe T, Ikeda H, Nagamori S, Wiriyasermkul P, Tanaka Y, Naka S, Kanai Y, Hagiwara K, Aoki M, Shimosegawa E, Kanai Y, Hatazawa J.  18F-FBPA as a tumor-specific probe of L-type amino acid transporter 1 (LAT1): a comparison study with 18F-FDG and 11C-Methionine PET.  Eur. J. Nucl. Med. Mol. Imaging  2017  44:321-331  PubMed ID: 27550420   DOI: 10.1007/s00259-016-3487-1

10133  Watabe T, Kanai Y, Ikeda H, Horitsugi G, Matsunaga K, Kato H, Isohashi K, Abe K, Shimosegawa E, Hatazawa J.  Quantitative evaluation of oxygen metabolism in the intratumoral hypoxia: 18F-fluoromisonidazole and 15O-labelled gases inhalation PET.  EJNMMI Res  2017  7:16  PubMed ID: 28210996   DOI: 10.1186/s13550-017-0263-6

10283  Watabe T, Hanaoka K, Naka S, Kanai Y, Ikeda H, Aoki M, Shimosegawa E, Kirihata M, Hatazawa J.  Practical calculation method to estimate the absolute boron concentration in tissues using 18F-FBPA PET.  Ann Nucl Med  2017  31:481-485  PubMed ID: 28439784   DOI: 10.1007/s12149-017-1172-5

9917  Podyma-Inoue KA, Moriwaki T, Rajapakshe AR, Terasawa K, Hara-Yokoyama M.  Characterization of Heparan Sulfate Proteoglycan-positive Recycling Endosomes Isolated from Glioma Cells.  Cancer Genomics Proteomics  2016  13:443-452  PubMed ID: 27807067   DOI: 10.21873/cgp.20007

7345  Yamaguchi A, Hanaoka H, Fujisawa Y, Zhao S, Suzue K, Morita A, Tominaga H, Higuchi T, Hisaeda H, Tsushima Y, Kuge Y, Iida Y.  Differentiation of malignant tumours from granulomas by using dynamic [(18)F]-fluoro-L-α-methyltyrosine positron emission tomography.  EJNMMI Res  2015  5:29  PubMed ID: 25977883   DOI: 10.1186/s13550-015-0109-z

9473  Koichi Fujikawa, Ryo Fukumori, Saki Nakamura, Takaya Kutsukake, Takeshi Takarada, Yukio Yoneda  Potential Interactions of Calcium-Sensitive Reagents With Zinc Ion in Different Cultured Cells  PLoS One  2015  10(5):e0127421  PubMed ID: 26010609   DOI: 10.1371/journal.pone.0127421

5999  Zhao S, Liu H, Liu Y, Wu J, Wang C, Hou X, Chen X, Yang G, Zhao L, Che H, Bi Y, Wang H, Peng F, Ai J.  miR-143 inhibits glycolysis and depletes stemness of glioblastoma stem-like cells.  Cancer Lett  2013  333(2):253-60  PubMed ID: 23376635   DOI: 10.1016/j.canlet.2013.01.039

15777  Ogawa K, Hirooka Y, Kishi T, Ide T, Sunagawa K.  Partially silencing brain toll-like receptor 4 prevents in part left ventricular remodeling with sympathoinhibition in rats with myocardial infarction-induced heart failure  PLoS One  2013  8(7):e69053  PubMed ID: 23874864   DOI: 10.1371/journal.pone.0069053

7999  Ogura M, Takarada T, Nakamichi N, Kawagoe H, Sako A, Nakazato R, Yoneda Y.  Exacerbated vulnerability to oxidative stress in astrocytic C6 glioma cells with stable overexpression of the glutamine transporter slc38a1.  Neurochem. Int.  2011  58:504-11  PubMed ID: 21219957   DOI: 10.1016/j.neuint.2011.01.007

18576  Kono S, Yoshida K, Tomosugi N, Terada T, Hamaya Y, Kanaoka S, Miyajima H.  Biological effects of mutant ceruloplasmin on hepcidin-mediated internalization of ferroportin  Biochim Biophys Acta.  2010  1802(11):968-75  PubMed ID: 20655381   DOI: 10.1016/j.bbadis.2010.07.011

9527  Shanlou Qiao, Weihua Li, Ryoko Tsubouchi, Keiko Murakami, Masataka Yoshino  Role of Vanilloid Receptors in the Capsaicin-Mediated Induction of iNOS in PC12 Cells  Neurochem Res . 2004 Apr;29(4):687-93  2004  29(4):687-93  PubMed ID: 15098930   DOI: 10.1023/b:nere.0000018839.59457.5c

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