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RCB2853 : WEHI-3B  update : 2018/08/02
CommentMouse cell line derived from myelomonocytic leukemia cells. BALB/c strain. TKG0584 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Warner, N.L.
Year of deposit 2008
Original cell TKG0584
Animal mouse < Mammals
Strain name BALB/c
Tissue myelomonocyte
Case history myelomonocytic leukemia cell lin
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0584)
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 6
User's Publication 1

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5288  Shimizu T, Izumi T, Seyama Y, Tadokoro K, Rådmark O, Samuelsson B.  Characterization of leukotriene A4 synthase from murine mast cells: evidence for its identity to arachidonate 5-lipoxygenase.  Proc Natl Acad Sci U S A  1986  83(12):4175-9  PubMed ID: 3012557
5287  McKean DJ, Infante AJ, Nilson A, Kimoto M, Fathman CG, Walker E, Warner N.  Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells.  J Exp Med  1981  154(5):1419-31  PubMed ID: 6170720
1294  Ralph P, Nakoinz I  Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture.  Cancer Res  1977  37:546-50  PubMed ID: 318922
716  Ralph P, Nakoinz I  Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.  J Immunol  1977  119:950-54  PubMed ID: 894031
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890
5289  Metcalf D, Moore MA, Warner NL.  Colony formation in vitro by myelomonocytic leukemic cells.  J Natl Cancer Inst  1969  43(4):983-1001  PubMed ID: 5259326

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User's Publication
9506  Nakaoku T, Kohno T, Araki M, Niho S, Chauhan R, Knowles PP, Tsuchihara K, Matsumoto S, Shimada Y, Mimaki S, Ishii G, Ichikawa H, Nagatoishi S, Tsumoto K, Okuno Y, Yoh K, McDonald NQ, Goto K.  A secondary RET mutation in the activation loop conferring resistance to vandetanib.  Nat Commun  2018  9:625  PubMed ID: 29434222

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