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RCB2812 : HeLa/Fucci  update : 2019/02/27
CommentA subline of the HeLa cell line expressing Fucci, a cell cycle marker. Cell line name : HeLa/Fucci #4.Important notice regarding nomenclature of Fucci.
Comment from the depositor
Terms and conditions1) A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2) In publishing the research results related to the BIOLOGICAL RESOURCE,a citation of the following literature is necessary. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S,Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. (2008) Visualizing Spatiotemporal Dynamics of Multicellular Cell Cycle Progression. Cell, 132:487-498. 3) The RECIPIENT agrees to acknowledge the DEPOSITOR in any publication, oral or written, reporting use of the BIOLOGICAL RESOURCE and provide a copy of the publication to the DEPOSITOR.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Miyawaki, Atsushi
Originator Miyawaki, Atsushi
Year of deposit 2008
Another name HeLa.S-Fucci
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Case history cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene Fucci
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 14


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Reference
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265 
4042  Sakaue-Sawano A1, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A.  Visualizing spatiotemporal dynamics of multicellular cell-cycle progression  Cell  2008  132:487-498  PubMed ID: 18267078

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User's Publication
7179  Izumi Y, Yamamoto S, Fujii K, Yokoya A.  Secondary Structure Alterations of Histones H2A and H2B in X-Irradiated Human Cancer Cells: Altered Histones Persist in Cells for at Least 24 Hours.  Radiat. Res.  2015  184:554-8  PubMed ID: 26488755
7394  Kaminaga K, Noguchi M, Narita A, Sakamoto Y, Kanari Y, Yokoya A.  Visualisation of cell cycle modifications by X-ray irradiation of single HeLa cells using fluorescent ubiquitination-based cell cycle indicators.  Radiat Prot Dosimetry  2015  166:91-4  PubMed ID: 25877544
7637  Kaida A, Miura M.  Visualizing the effect of hypoxia on fluorescence kinetics in living HeLa cells using the fluorescent ubiquitination-based cell cycle indicator (Fucci).  Exp. Cell Res.  2011  318:288-97  PubMed ID: 22079518
7770  Nakayama M, Kaida A, Deguchi S, Sakaguchi K, Miura M.  Radiosensitivity of early and late M-phase HeLa cells isolated by a combination of fluorescent ubiquitination-based cell cycle indicator (Fucci) and mitotic shake-off.  Radiat. Res.  2011  176:407-11  PubMed ID: 21692656
9787  Adamowicz M, Vermezovic J, d'Adda di Fagagna F.  NOTCH1 Inhibits Activation of ATM by Impairing the Formation of an ATM-FOXO3a-KAT5/Tip60 Complex.  Cell Rep  2016  16:2068-2076  PubMed ID: 27524627
9857  Izumi Y, Fujii K, Yamamoto S, Matsuo K, Namatame H, Taniguchi M, Yokoya A.  DNA damage response induces structural alterations in histone H3-H4.  J. Radiat. Res.  2017  58:59-65  PubMed ID: 27672100
10101  Kobayashi D, Kakinouchi K, Nagae T, Nagai T, Shimura K, Hazama A.  Cesium reversibly suppresses HeLa cell proliferation by inhibiting cellular metabolism.  FEBS Lett.  2017  591:718-727  PubMed ID: 28134446
10655  Boström J, Sramkova Z, Salašová A, Johard H, Mahdessian D, Fedr R, Marks C, Medalová J, Souček K, Lundberg E, Linnarsson S, Bryja V, Sekyrova P, Altun M, Andäng M.  Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells.  PLoS ONE  2017  12:e0188772  PubMed ID: 29228002
10709  Mita P, Wudzinska A, Sun X, Andrade J, Nayak S, Kahler DJ, Badri S, LaCava J, Ueberheide B, Yun CY, Fenyö D, Boeke JD.  LINE-1 protein localization and functional dynamics during the cell cycle.  Elife  2018  7:  PubMed ID: 29309036
10978    Effect of metal–metal distance in Ni(II)-based metallo-supramolecular polymers: DNA binding and cytotoxicity  RSC Adv  2017  7, 38008-38013 
11174  Manila NG, Kaida A, Nakahama KI, Miura M.  Insulin-like growth factor I receptor regulates the radiation-induced G2/M checkpoint in HeLa cells.  Biochem. Biophys. Res. Commun.  2018    PubMed ID: 30119887
11230  Li SC, Stucky A, Chen X, Kabeer MH, Loudon WG, Plant AS, Torno L, Nangia CS, Cai J, Zhang G, Zhong JF.  Single-cell transcriptomes reveal the mechanism for a breast cancer prognostic gene panel.  Oncotarget  2018    PubMed ID: 30279960
11348  D'Alessandro G, Whelan DR, Howard SM, Vitelli V, Renaudin X, Adamowicz M, Iannelli F, Jones-Weinert CW, Lee M, Matti V, Lee WTC, Morten MJ, Venkitaraman AR, Cejka P, Rothenberg E, d'Adda di Fagagna F.  BRCA2 controls DNA:RNA hybrid level at DSBs by mediating RNase H2 recruitment.  Nat Commun  2018    PubMed ID: 30560944
4090  B. Cameron, G. Traver, M.L. Freeman, J. Wang, K. Sekhar, L. Johnson, D. Dean, M. Fahhoum  Intrinsic Resistance of V-SVZ Neural Stem Cells Subjected to Concurrent Daily Radiation and Temozolomide Therapy  International Journal of Radiation Oncology  2018  102:e145 



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