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Cell No. : Cell Name
RCB2783 : RGC-6  update : 2018/08/02
CommentRat cell line derived from glioma induced by N-nitrosomethylurea. TKG0242 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Benda
Year of deposit 2008
Original cell TKG0242
Animal rat < Mammals
Tissue brain
Classification cancer
History Sato, Y. → Cell Resource Center for Biomedical Research, Tohoku University(TKG0242)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, AST
Images
deposit info
lot info
Reference information Reference 3
User's Publication 1


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Reference
5272  Bondareff W, Tuttle RL.  Cortical cytoplasm and configurational changes of RGC-6 cells exposed to cytochalasin B.   Am J Anat  1975  144(3):257-73  PubMed ID: 1211367
5271  Dawson G, Kemp SF, Stoolmiller AC, Dorfman A, Kennedy JP Jr.  Biosynthesis of glycosphingolipids by mouse neuroblastoma (NB41A), rat glia (RGC-6) and human glia (CHB-4) in cell culture.  Biochem Biophys Res Commun  1971  44(3):687-94  PubMed ID: 5166263
645  Benda P, Lightbody J, Sato G, Levine L, Sweet W.  Differentiated rat glial cell strain in tissue culture.  Science  1968  161(3839):370-1  PubMed ID: 4873531

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User's Publication
6928  Aoki M, Watabe T, Nagamori S, Naka S, Ikeda H, Kongpracha P, Horitsugi G, Kanai Y, Shimosegawa E, Kanai Y, Hatazawa J.  Distribution of LAT1-targeting PET tracer was independent of the tumor blood flow in rat xenograft models of C6 glioma and MIA PaCa-2.  Ann Nucl Med  2019  33(6):394-403  PubMed ID: 30820863



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